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Native and in vitro associated phycobilisomes of nostoc sp composition energy transfer and effect of antibodies


, : Native and in vitro associated phycobilisomes of nostoc sp composition energy transfer and effect of antibodies. Biochimica et Biophysica Acta 723(3): 340-349

The relationship of the structure and function of the light-harvesting antennae in the blue-green alga Nostoc sp. was further elucidated by reconstitution experiments. Separated phycoerythrin-phycocyanin complexes and allophycocyanin fractions were reassociated as described earlier (Canaani, et. al. (1980) into functional phycobilisomes with a 70% yield. Native and reassociated phycobilisomes had molar ratios of about 1.4:1.1:1.0 of phycoerythrin:phycocyanin:allophycocyanin. Energy transfer was demonstrated by their fluorescence emission maximum at .apprx. 675 nm (20.degree. C), and their excitation spectra (emission wavelength 680 nm) which reflected the contribution of the three constitutive phycobiliproteins. Scans of Coomassie blue-stained SDS [sodium dodecyl sulfate] polyacrylamide gels showed that the polypeptide composition of native and reassociated phycobilisomes was virtually indistinguishable. Reassociation of phycobilisomes was dependent on the interaction of allophycocyanin and phycocyanin, because it could be blocked with antisera to phycocyanin and allophycocyanin, but not to phycoerythrin. In addition, reassociation did not occur when a 31,000 Da [dalton] polypeptide, which is part of the phycoerythrin-phycocyanin complex, was reduced in size (by 4000 Da). Apparently, at least 2 domains are required for functional reassociation of phycobilisomes involving phycocyanin and allophycocyanin.

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