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Native and inoculated rhizobia isolated from field grown phaseolus vulgaris effects of liming an acid soil on antibiotic resistance


, : Native and inoculated rhizobia isolated from field grown phaseolus vulgaris effects of liming an acid soil on antibiotic resistance. Soil Biology & Biochemistry 19(2): 179-186

An investigation was made to determine the tolerance to growth in acid culture medium (pH 4.6), and to several antibiotics, of rhizobia isolated from Phaseolus vulgaris plants grown in limed and unlimed field plots [Brazil]. The plants were originally inoculated with Rhizobium strain CO5 and isolates were made after 5 successive crops of beans (only inoculated at the first crop) during a 2 yr period, and simultaneously from plots planted to beans for the first time. Rhizobium isolates identified as CO5 using immunodiffusion were all found to be identical to the original CO5 strain in their tolerance to acidity and resistance to 7 antibiotics. However, the isolates of native soil rhizobia from unlimed plots were found to be more tolerant to acid conditions and less tolerant to chloramphenicol and kanamycin than those isolated from limed plots. The differences in antibiotic resistance between lime treatments was greater in isolates from plots planted 5 times with beans than in those taken from plots never planted to this crop. The antibiotic resistant Rhizobium isolates, mainly originating from limed plots, showed predominantly large, flat, wet, translucent colonies on yeast mannitol agar (YMA) plates. The antibiotic sensitive isolates, mainly originating from unlimed plots, showed predominantly small, dry, opaque, circular colonies on YMA plates. The results confirm the in vitro evaluation of acid tolerance reflects the ability of Rhizobium strains to nodulate legumes growing in acids soil conditions. That significant antibiotic production may occur in the plant-soil system is considered as an explanation for the observed differences in antibiotic resistance between native rhizobia isolated from limed and unlimed plots.

US$19.90

DOI: 10.1016/0038-0717(87)90079-4


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