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Native and modified low density lipoprotein interaction with human platelets in normal and homozygous familial hypercholesterolemic subjects

, : Native and modified low density lipoprotein interaction with human platelets in normal and homozygous familial hypercholesterolemic subjects. Biochemical Journal 224(1): 13-20

The binding of low density lipoproteins (LDL) as well as LDL modified by cyclohexanedione (CHD-LDL) to gel-filtered platelets (GFP), and its effect on platelet function were studied in normal and in homozygous familial hypercholesterolemic (HFH) subjects [plays a major role in atherosclerosis]. Only normal-derived LDL could significantly compete with normal 125I-labeled LDL for binding to normal platelets. When GFP from normal subjects were incubated with normal LDL at concentrations of 25-200 .mu.g of protein/ml, platelet aggregation in the presence of thrombin (0.5 IU/ml) was increased by 65-186%. CHD-LDL, at similar concentrations, caused the opposite effect and decreased platelet aggregation by 26-47%. Both LDL and CHD-LDL (100 .mu.g/ml) from HFH patients, when incubated with normal GFP, caused a significant reduction in platelet aggregation (33 and 50%, respectively). When HFH-derived platelets were used, both patient LDL and CHD-LDL (but not the normal lipoprotein) could markedly compete with the patient 125I-labeled LDL for binding to the platelets. LDL and CHD-LDL (100 .mu.g/ml) from normal subjects decreased aggregation of HFH-platelets by 52 and 85%, respectively, while corresponding concentrations of LDL derived from HFH subjects (HFH-LDL) and CHD-LDL derived from HFH subjects (CHD-HFH-LDH) increased platelet aggregation by 165 and 65%, respectively. The following conclusions are supported: platelet activation by LDL in normal subjects is through the arginine-rich apoprotein-binding site; more than one binding site for LDL exists on platelets; under certain circumstances, LDL binding can cause a reduction in platelet activity; specificity for LDL binding to the platelets resides in different regions of the lipoprotein in HFH and in normal subjects. A model for LDL-platelet interaction in normal and in HFH subjects was suggested.


PMID: 6508753

Other references

Gane, E., 2005: Treatment of chronic hepatitis C in patients with renal disease. Medical Journal of Malaysia 60 Suppl B: 72-76

Oberlander, H.; Ferkovich, SM., 1980: The control of chitin biosynthesis in Plodia interpunctella. International Congress of Entomology Proceedings, 16: 65

Rao, A.S.; Nalesnik, M.; Elders, E.; Rowe, D.; Rybka, W.; Zeevi, A.; Whiteside, T.; Pham, S.; Keenan, R.; Griffith, B.; Fung, J.J.; Starzl, T.E., 1996: Cellular immunotherapy for post-transplant lymphoproliferative disease using recipients lymphokine activated killer cells generated ex-vivo. Cell Transplantation 5(5 SUPPL 2): 79

Akhilesh Singh; Singh, D.V., 1994: In vitro and in vivo efficacy of chemicals against Streptomyces aureofaciens causing common scab of potato. The best control of S. aureofaciens was given by agallol-3 [methoxyethylmercury chloride], followed by Emisan-6 [2-methoxyethylmercury chloride] and streptocycline, in pot trials with 18 fungicides and antibiotics.

Rollan, A.; Figueroa, C.; Giancaspero, R.; Muller, I.; Opazo, P.; Hevia, E., 1997: Host and bacterial determinants of the consequences of Helicobacter pylori infection. Gastroenterology 112(4 SUPPL ): A271

Archer, Michael, 2004: Wasps and bees found on Tresco, Isles of Scilly. Entomologist's Monthly Magazine 140(1683-84): 232, August

Wolf, S., 1981: Bullae formation from a contoured stethoscope. Anesthesiology 54(6): 521-522

Scholander, P.F.; Hammel, H.T.; Hemmingsen, E.A.; Bradstreet, E.D., 1964: Hydrostatic Pressure And Osmotic Potential In Leaves Of Mangroves And Some Other Plants. Describes experiments on Rhizophora mangle, Laguncularia racemosa, Avicennia nitida, Batis maritima, and Salicornia pacifica, and also on Distichlis spicata and Larrea divaricata, using an external gas-pressure technique. Chief conclusions were th...

Sharma, R.C.; Ali, R., 1976: The effect of ultraviolet irradiation on tyrosinase. Radiation Research 66(1): 33-41

Stewart, S.; Murray, S.B.; Skull, S.A., 2003: Evaluation of health-care worker vaccination in a tertiary Australian hospital. Maintaining a complete vaccination status for health-care workers (HCWs) is important to minimize morbidity among staff and patients. Despite recommendations from public-health authorities to support this process, not all hospitals have adequate p...