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Nephelometric and turbidometric assays of cellulase activity

, : Nephelometric and turbidometric assays of cellulase activity. Analytical Biochemistry 116(1): 133-136

Extensively ball-milled cellulose fibers were used as natural substrate for the determination of [Trichoderma reesei] cellulase activity. This physical treatment breaks the large cellulose fibers to small but insoluble particles yielding a substrate accessible for complete enzymatic breakdown. The parameters studied to estimate the activity of cellulases were the decrease in optical density of ball-milled suspension of fibers and simultaneous measurement of liberated sugars during hydrolysis. A good correlation was found between the initial rate of reaction and the amount of sugar released at given times.


PMID: 7197888

DOI: 10.1016/0003-2697(81)90334-1

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