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Note on comparative efficiencies of sulfur 35 fertilizer labeling and soil labeling techniques for determining the uptake of applied sulfur

, : Note on comparative efficiencies of sulfur 35 fertilizer labeling and soil labeling techniques for determining the uptake of applied sulfur. Indian Journal of Agricultural Sciences 45(8): 385-388

A greenhouse experiment was conducted in pots, using 'Bragg' soybean (Glycine max (L.) Merr.) as the test crop and CaSO4 .cntdot. H2O, (NH4)2SO4 and Na2SO4. In the fertilizer-labeled series the fertilizer was tagged with 35S at specific activity of 0.25 mCi/g S. In the soil-labeled series 450 g of soil were tagged with 0.450 mCi 35S with wetting, mixing and drying cycles, and 10 g lots of the labeled soil were mixed with the soil of each pot along with unlabeled S fertilizer. In the plants the percentage of S absorbed from the fertilizer in the fertilizer-labeled series was calculated from the ratio of specific activity of S in the plant and in the labeled fertilizer standard. In the soil-labeled series the specific activity of the plant grown without S was taken as the standard. In the fertilizer-labeled series the S uptake increased with successive levels of applied S from all sources under test, except with 20 ppm S as Na2SO4, which slightly depressed the S uptake over its 10 ppm level. Of the 3 S sources, Na2SO4 contributed the lowest percentage of fertilizer S in the nutrition of soybean plants; this treatment also depressed the dry matter yield. Absorption of fertilizer S increased in pots treated with CaSO4 .cntdot. 2H2O or (NH2)SO4. In the plants of the soil-labeled series the percentage S derived from the fertilizer increased with successive levels of S from (NH4)2SO4 only; the values decreased with Na2SO4. The percentage of fertilizer uptake in the soil-labeled series was higher than in the fertilizer-labeled series; the order of performance of different sources for S supply as determined by the soil-labeling method was not the same as found with the fertilizer-labeling method. The response of dry matter yield of the fertilizer-labeled series due to applied S is supported by radio-assay data, but the data obtained from the soil-labeled series do not corroborate the yield responses. In evaluating the efficiency of applied S for determining the uptake of S from the fertilizer, as isolated from the native soil souce, the soil-tagging method is less satisfactory than the fertilizer-tagging method.


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