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On the tyrosinate involvement in the schiff base de protonation in the proton pump cycle of bacterio rhod opsin


, : On the tyrosinate involvement in the schiff base de protonation in the proton pump cycle of bacterio rhod opsin. Photochemistry & Photobiology 39(1): 75-80

The UV transient absorption assigned to the tyrosinate species in bacteriorhodopsin [from Halobacterium halobium] is followed in time and as a function of pH. Both its rise time and titration curve closely resemble those observed for the production of the M412 intermediate. These results may support a recently proposed mechanism that couples tyrosinate production to the Schiff base deprotonation in the proton pump of bacteriorhodopsin.

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