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Partial characterization of herpes simplex virus type 2 specific poly adenylic acid plus rna by hybridization of eco r i generated herpes simplex virus type 2 dna fragments


, : Partial characterization of herpes simplex virus type 2 specific poly adenylic acid plus rna by hybridization of eco r i generated herpes simplex virus type 2 dna fragments. Virology 92(2): 507-517

Polyadenylated RNA appearing in polyribosomes from herpes simplex virus type 2 (HSV-2)-infected [rabbit kidney and African green monkey kidney Vero] cells was isolated at different stages of infection. The amounts of virus-specific RNA hybridizing to individual EcoRI fragments of HSV-2 DNA were detected. Templates coding for early virus-specific RNA are widely dispersed within the HSV-2 genome in the short and long segment since each of the EcoRI fragments showed hybridization with early isolated poly(A)+ RNA. The virus-specific fraction of labeled poly(A)+ RNA which hybridized to each of the EcoRI fragments increased with 1-.beta.-D-arabinofuranosylcytosine-treated (Ara-C) poly(A)+ RNA and with late poly(A)+ RNA. Poly(A)+ RNA from infected, Ara-C treated cells contained virus-specific RNA which hybridized to each of the EcoRI fragments. The poly(A)+ RNA, specific for 1 EcoRI fragment, varied with the stage of infection, comparing it to other fragment-specific poly(A)+ RNA.

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