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Patch clamping the outer mitochondrial membrane

, : Patch clamping the outer mitochondrial membrane. Journal of Membrane Biology 97(1): 21-30

Intact giant mitochondria isolated from the liver of mice fed a diet containing cuprizone were studied using patch microelectrodes. The current-voltage curves were nonlinear, suggesting the presence of voltage-sensitive channels. In the negative range of voltage, the channels appear to close with increasing magnitude of the voltage. The dependence of the conductance on voltage is similar to that of the outer membrane channels (VDAC) studied in planar bilayers. Occasionally, over a narrow range of positive potentials, the conductance also decreases as in the bilayer studies. However, more frequently the conductance increases sharply in a completely reversible manner at potentials greater than 10 to 20 mV. The increase in conductance with voltage may be interpreted as a major rearrangement of membrane components. Qualitatively comparable results were obtained using fused outer membranes isolated from Neurospora mitochondria. The behavior of VDAC is affected by treatment with succinic anhydride or the polyanion, polymethacrylate, maleate, styrene (12:3). We have found similar effects in the negative range of potentials in patches from giant mitochondria treated in the same fashion.


PMID: 3612769

DOI: 10.1007/bf01869611

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Crouzel C.; Langstrom B.; Pike V.W.; Coenen H.H., 1987: Recommendations for a practical production of carbon 11 methyl iodide. The application of these recommendations should permit [11C]methyl iodide to be obtained within 10 min after the end of the bombardment of target gas. The decay-corrected radiochemical yield from [11C]carbon dioxide would be expected to be 80% and...

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Van Zwieten, P.A., 1988: Pharmacology of the alpha 2-adrenoceptor agonist rilmenidine. Most alpha 2-adrenoceptor agonists developed so far will penetrate into the brain, thus causing central hypotensive activity, mediated by the stimulation of alpha 2 adrenoceptors in the region of the nucleus tractus solitarii, the vasomotor center...

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