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Poly ethylene glycol induced transplantation of chloroplasts into protoplasts an ultrastructural assessment


, : Poly ethylene glycol induced transplantation of chloroplasts into protoplasts an ultrastructural assessment. Plant Science Letters 7(1): 7-16

Mixtures of protoplasts from suspension-cultured cells of Parthenocissus tricuspidata and chloroplasts from Petunia hybrida were treated with polyethylene glycol (PEG). The envelope of intact chloroplasts readily fused with the protoplast plasmalemma to establish continuity between the chloroplast stroma and the protoplast cytoplasm. Broken chloroplasts became localized in membrane-bounded vesicles in the cytoplasm of the higher plant protoplasts.

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