+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Preferential homing of passively transferred thymus derived cells into skin allo grafts of mice

, : Preferential homing of passively transferred thymus derived cells into skin allo grafts of mice. Transplantation (Baltimore) 28(3): 247-252

An assay which uses 2 differentially labeled cell populations was used to characterize the preferential localization of passively transferred syngeneic cells immunized to specific alloantigens. Splenocytes cytotoxic to B10.D2 and B10.BR alloantigens were harvested from (C57BL/6 .times. A/J)F1 (B6AF1) donors bearing acutely rejected skin allografts. One population was labeled in vitro with 3H-thymidine and the other with 14C-thymidine. The labeled cells were pooled and then transferred i.v. into B6AF1 hosts bearing 5 day old skin grafts from B10.D2 and B10.BR donors. After 48 h the mice were killed, and the relative amount of cells present in the skin grafts and draining axillary lymph nodes was derived by comparing the 3H:14C ratios of the harvested tissues. Cytotoxic splenocytes harvested from donors bearing acutely rejected skin allografts apparently preferentially localize to the relevant skin allograft after passive systemic transfer. The homing behavior of these splenocytes was augmented by T [thymus-derived] cell enrichment and significantly diminished by pretreatment with anti-Thy-1.2 serum plus rabbit complement. There was no evidence of preferential homing within the draining axillary lymph nodes. A T cell population derived from in vivo sensitized splenocytes exhibits preferential homing to relevant skin allografts upon passive transfer.


Other references

Wells, M.E.; Hefley, P.J., 1974: The effect of length of equilibration time on sperm cells stored in straws. Ejaculates from mature bulls were processed using standard procedures requiring the addition of gycerol to the extender. Each ejaculate was split and the 2 fractions then exposed to glycerol for either 4 or 18 h prior to freezing.Ejaculates after...

Ulrich,W.; Komosinski,K.; Zalewski,M., 2008: Body size and biomass distributions of carrion visiting beetles do cities host smaller species?. The question how animal body size changes along urban-rural gradients has received much attention from carabidologists, who noticed that cities harbour smaller species than natural sites. For Carabidae this pattern is frequently connected with inc...

Anonymous, 1982: Give cyclists room to move. British Medical Journal 285(6335): 86-86

Lucianez, M.-Jose; Carlos-Simon, J., 1992: Evolution of chaetotaxy in Fissuraphorura gisini (Collembola: Tullbergiinae). The morphology and chaetotaxy of juvenile instars in Fissuraphorura gisini (Selga, 1966) are described. The postembryonic development of the postantennal organ and sensorial organ in antennal segment III results very interesting from the phylogene...

de Vries, L.; Phillip, M., 2011: Role of pelvic ultrasound in girls with precocious puberty. Pelvic ultrasound evaluation of girls presenting with secondary sex characteristics before the age of 8 years may help to differentiate precocious puberty from premature thelarche, functioning ovarian cyst, and obesity. However, although uterine a...

Lukomskiĭ, I.I., 1973: Psychic alterations in Gayet-Wernicke's alcoholic encephalopathy. Zhurnal Nevropatologii i Psikhiatrii Imeni S.S. Korsakova 73(8): 1180-1185

Flessner, M.F., 2006: Peritoneal ultrafiltration: mechanisms and measures. The net ultrafiltration during peritoneal dialysis equals the osmotic ultrafiltration minus the fluid loss. Fluid loss is made up primarily of hydrostatic pressure-driven convection to the tissues surrounding the peritoneal cavity and a lesser amo...

Read, B.; Luckett, D.; Smithard, R.; Brown, A., 1998: Register of Australian winter cereal cultivars, Hordeum vulgare (barley) cv. Tantangara. Reg. No. AUS 499034, Registered 26 July 1996. Australian journal of experimental agriculture8(2): 207

Pilalis, E.; Koutsandreas, T.; Valavanis, I.; Athanasiadis, E.; Spyrou, G.; Chatziioannou, A., 2016: KENeV: A web-application for the automated reconstruction and visualization of the enriched metabolic and signaling super-pathways deriving from genomic experiments. Gene expression analysis, using high throughput genomic technologies,has become an indispensable step for the meaningful interpretation of the underlying molecular complexity, which shapes the phenotypic manifestation of the investigated biologica...

Sacharczuk, M.; Jaszczak, K., 1999: Preliminary study of Ag-NOR and C-band in high and low analgesia mice. Cytogenetics & Cell Genetics 85(1-2): 96