+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Proteases as catalysts for enzymic syntheses of opioid peptides

, : Proteases as catalysts for enzymic syntheses of opioid peptides. Journal of Biological Chemistry 255(17): 8234-8238

Two alternative pathways leading to protease-mediated syntheses of Leu- and Met-enkephalin are described. Each peptide bond of the opiate peptides was formed by either papain, or alpha-chymotrypsin catalysis. N alpha-t-Butyloxycarbonyl amino acids and peptides or their esters served as carboxyl components, whereas amino acid and peptide phenylhydrazides were used as acceptor nucleophiles. After removal of the protecting groups, the free pentapeptides were purified to homogeneity. They exhibited naloxone-reversible opiate like activity in guinea pig ileum and mouse vas deferens assays. The present study indicates that enzymic synthesis is a useful tool for rapid preparation of homogeneous peptides with highest obtainable optical purity.


PMID: 7410361

Other references

Matsunaga, R.; Shimano, R., 2012: Nonequilibrium BCS state dynamics induced by intense terahertz pulses in a superconducting NbN film. Using terahertz (THz) pump-THz probe spectroscopy, we have investigated the dynamics of the nonequilibrium BCS state in a superconducting NbN film after the impulsive photoinjection of high-density Bogoliubov quasiparticles. The superconducting st...

Fregoni, M., 1981: A new rootstock Fercal, available soon in Italy. Vignevini 8(11): 13-15

Braicu, C.; Mehterov, N.; Vladimirov, B.; Sarafian, V.; Nabavi, S.Mohammad.; Atanasov, A.G.; Berindan-Neagoe, I., 2017: Nutrigenomics in cancer: Revisiting the effects of natural compounds. Nutrigenomics effects have an important role in the manipulation of dietary components for human benefit, particularly in cancer prevention or treatment. The impact of dietary components, including phytochemicals, is largely studied by nutrigenomi...

Heil T.P.; Lindsay R.C., 1988: Volatile compounds in flavor tainted fish from the upper wisconsin usa river. Fillets from Walleye pike (Stizostedion vitreum) and Northern pike (Esox lucius) captured from the Upper Wisconsin River were analyzed for volatile components using simultaneous steam distillation-extraction and GC-MS techniques. Based on comparat...

Willis, J.H.; Minior, T.; Gonnelli, R., 2002: Injection devices. Injection devices, such as needleless injection devices, and methods of making and using the same are disclosed.

Numazaki, K.; Umetsu, M.; Chiba, S., 1994: Effect of glycyrrhizin in children with liver dysfunction associated with cytomegalovirus infection. Glycyrrhizin (GL) has an inhibitory effect on several viruses including human immunodeficiency virus type 1 (HIV-1) and varicella-zoster virus (VZV). In addition, some therapeutic and prophylactic effects on chronic active viral hepatitis have bee...

Fukutomi, T.; Zhou, Y.; Kawai, S.; Eguchi, H.; Wands, J.R.; Li, J., 2005: Hepatitis C virus core protein stimulates hepatocyte growth: correlation with upregulation of wnt-1 expression. Hepatitis C virus (HCV) core protein has been implicated in the development of human hepatocellular carcinoma (HCC). Here we report that expression of HCV core protein by transient transfection increased cell proliferation, DNA synthesis, and cell...

Chai, H.; Fang, Q.; Huang, X.; Zhou, Y.; Luo, Y., 2015: Prenatal management and outcomes in mirror syndrome associated with twin-twin transfusion syndrome. The aim of this article is to investigate the prevalence, clinical presentation, prenatal management, and prognosis of mirror syndrome associated with twin-twin transfusion syndrome (TTTS) treated by amnioreduction or selective fetocide. A retrosp...

Kagawa, Y., 1978: Medical and ecological survey o. Eiyogaku zasshi Japanese journal of nutrition 36(1): 19-30

Robinson, A.; Gibson, A., 1917: Description of a reconstruction model of a horse embryo twenty-one days old. Edinburgh Transactions of the Royal Society, 51 331-346