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Quantitative determination of the superoxide radicals in the xanthine oxidase reaction by measurement of the esr signal of the superoxide radical spin adduct of 5 5 dimethyl 1 pyrroline 1 oxide


, : Quantitative determination of the superoxide radicals in the xanthine oxidase reaction by measurement of the esr signal of the superoxide radical spin adduct of 5 5 dimethyl 1 pyrroline 1 oxide. Journal of Pharmacobio-Dynamics 7(8): 563-569

Reactivities of biologically active compounds with [carcinogenic] superoxide radicals were examined by measurement of ESR spectra of 5-hydroperoxy-2,2-dimethyl-1-pyrrolidinyloxyl formed from 5,5-dimethyl-1-pyrroline-1-oxide and the superoxide radicals generated by xanthine-xanthine oxidase system. Quantitative determination of the superoxide radicals was performed by comparing the intensity of the spin adducts with that of 4-hydroxy-2,2,6,6-tetramethyl-piperidinooxyl whose spin concentration was determined. Methyl viologen enhanced the formation of this spin adducts, while rutin and luteoskyrin prevented it.

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