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Reduced emergence of acycloguanosine resistant hsv2 strains in an in vitro model

, : Reduced emergence of acycloguanosine resistant hsv2 strains in an in vitro model. Annales de l'Institut Pasteur Virology 138(4): 479-490

Acycloguanosine-resistant (ACG-R) thymidine-kinase-deficient (TK-) mutants are present among acycloguanosine-sensitive (ACG-S) viruses in herpes simplex virus (HSV) populations, but they have a limited capacity to emerge under ACG treatment in clinical practice. An in vitro model of mixed infection of Vero cell cultures is described, in which virus production with or without 25 .mu.M ACG was measured by an infectious centre assay. Four virus strains were used in the mixed infection experiments. Cloned ACG-S TK+ and ACG-R TK- were obtained prior to any treatment from the lesions of a patient with severe genital herpes. HSV2 derived from the previous ACG-R TK- strain after passaging under increasing concentrations of phosphonoformic acid (PFA) up to 600 .mu.g/ml (HSV2 ACG-R/PFA) and a mumps virus were also used. A reduced emergence of the ACG-R TK- HSV2 strain was observed in the mixed infection with the ACG-S TK+ HSV2 strain under ACG; it was more obvious when the HSV2 ACG-S component was higher than the HSV2 ACG-R component. In addition, a reduced emergence under ACG of the ACG-R component was observed in mixed infections involving the previous ACG-S TK+ HSV2 strain with either the HSV2 ACG-R/PFA or the mumps virus as ACG-R partner. These results indicate that a reduced emergence of the ACG-R strain in mixed infections with ACG-sensitivie HSV2 can be observed under ACG, whether or not the ACG-R strain provides an appropriate target for the ACG triphosphate-activated form. This might suggest a non-specific mechanism for the reduced emergence of HSV-resistant strains in mixed populations.


Other references

Stone, M.J., 2007: History of site-tumor conferences at Baylor Sammons Cancer Center. Proceedings 19(3): 249-249

Kalita, S.R., 1930: Material zur Erforschung des Zooplanktons der Alte Kuban bei Krasnodar (Stadt im Nord-Kaukasua). Resume. Travaux de la Station Biologique du Caucase du Nord, 8: 109

Gage, B.F.; Zahid, M.; Mcmullin, T.; Doggette, A.L.; Goldstein, N.; Shry, E., 1997: Watchful waiting versus vitamin K-1 in overanticoagulated non-bleeding patients A retrospective study. Jgim 12(Suppl 1): 71

Frances, W.W., 1959: Dr. William Willoughby FRANCES (1878-1959). Mcgill Medical Journal 28: 123-126

Anonymous, 1981: FDA approves more medical devices. JAMA 246(18): 2014-2014

Przybyla, C.P.; Musinski, W.D.; Castelluccio, G.M.; McDowell, D.L., 2013: Microstructure-Sensitive HCF and VHCF Simulations. This paper provides some background and historical review of how microstructure-sensitive finite element simulations can play a role in understanding the effects of stress amplitude, R-ratio, and microstructure on fatigue crack formation and early...

Wuest, J.D.; Madonik, A.M.; Gordon, D.C., 1977: Vinyl ketenes synthesis of dextro actinidine. Dehydrochlorination of 2,6,6-trimethylcyclohex-2-ene-1-carbonyl chloride yielded 2,6,6-trimethyl-1-carbonylcyclohex-2-ene, a vinylketene which was isolated and characterized. Dehydrochlorination of (1S,5R)-5-methyl-2-(1-methylethylidene)cyclopenta...

Juhel-Gaugain, M.; Anger, B.; Laurentie, M., 1999: Multiresidue chromatographic method for the determination of macrolide residues in muscle by high-performance liquid chromatography with UV detection. A high-performance liquid chromatographic (HPLC) method for the simultaneous determination of tilmicosin, tylosin, spiramycin, and its major metabolite neospiramycin was developed that is suitable for porcine, bovine, and poultry muscles. Macrolid...

Braden, M.L.; Marble, R.A., 1995: Method for clarifying wastewater containing surfactants. Official Gazette of the United States Patent & Trademark Office Patents 1176(3): 1799, July 18

Zschoch, H.; Kober, B., 1967: Statistical analysis of metastasising bronchial carcinoma based on autopsy studies. Archiv für Geschwulstforschung 30(2): 126-134