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Replication of Heliothis zea baculovirus in an insect cell line

, : Replication of Heliothis zea baculovirus in an insect cell line. Intervirology 16(2): 71-79

The baculovirus, Heliothis zea nuclear polyhedrosis virus (HzSNPV), was grown in the H. zea cell line, IPLB-1075. Light microscopy observations showed that 95% of inoculated cells had CPE by 6 days postinoculation (p.i.). Growth curves of intracellular and extracellular virus showed that maximum titers (TCID50 units) were reached 3-4 days p.i. Studies on HzSNPV morphogenesis revealed that ultrastructural events were typical of baculovirus replication in vitro. EcoRI restriction enzyme analyses of viral DNA produced in larvae and in cell culture were identical and indicated that this virus was HzSNPV.


PMID: 6276328

Other references

Wellemans, G.L.unen, J.L.chesinger, E.B.ondeel, H., 1970: Respiration troubles of cattle: isolation of a virus (220. Annales de medecine veterinaire: 114 (2) 89-93

Huang Jie.; Cai Shengli.; Song Xiaoling.; Wang Chongming.; Y.J.a.; Yang Conghai., 1995: Study on artificial infection for Penaeus chinensis by the pathogen of the explosive epidemic disease of shrimp. Marine Fisheries Research (Shandong). Aug; 161: 51-58

Akiyama, H.; Miyahara, M.; Toyoda, M.; Saito, Y., 1995: Liquid chromatographic determination of fumonisins B-1 and B-2 in corn by precolumn derivatization with 4-(N,N-dimethylaminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole (DBD-F). A new, sensitive and rapid method has been developed for the analysis of fumonisin B-1 and fumonisin B-2 in corn. Fumonisin B-1 and fumonisin B-2 which are the major metabolites produced by the fungi Fusarium moniliforme and Fusarium proliferatum,...

Kim, J.K.; Chang, D.G., 1990: Studies on the reproductive efficiency and causes of reproductive disorders in cheju pony mares i. a study on the reproductive performance in cheju pony mares. The reproductive status of 428 heads of Cheju pony mares raised on Cheju island was investigated to improve the reproductive efficiency and to get fundamental data for Cheju pony mares. 1. The average ages at the first estrus and the first foaling...

Gruesser O J., 1972: Meta contrast and the perception of the visual world. Pfluegers Archiv European Journal of Physiology 332(SUPPL): R98

Kovacs, K.; Lydersen, C.; Hammill, M., 1996: Scientific correspondence Grey seal Cannibalism. Marine Mammal Science 12(1): 161

Castaing, J.; Cambeilh, D., 2000: Assessment of the necessity to adjust cystine supply in a maize-soybean diet for weaned piglets. A low cystine (24% cystine/lysine) post-weaning maize-soyabean diet, containing 18% protein and adequate in lysine, methionine, threonine, tryptophan, isoleucine and valine, was compared with a control diet containing 21% crude protein and 27% cys...

Masuda, Y.; Iwama, T.; Yamashita, T.; Sakai, T.; Hibi, M.; Tanaka, S.; Shigenobu, K.; Kasuya, Y., 1991: Vasorelaxing and receptor binding properties of NZ-105, a novel dihydropyridine derivative, in isolated rabbit aorta. The vasorelaxing and dihydropyridine receptor binding properties of NZ-105, a new dihydropyridine derivative, were studied using isolated rabbit aorta, and compared with those of nicardipine, nifedipine and diltiazem. NZ-105 (3 x 10(-10)-3 x 10(-9...

Frisch H.; Parth K.; Schober E.; Swoboda W., 1978: Circadian levels of 17 hydroxy progesterone testosterone and cortisol in children with congenital adrenal hyperplasia with and without treatment. Pediatric Research 12(11): 1084

Koehn, R.K.; Rossi, J.; Stein, C.A., 2001: In defense of antisense. Science 293(5532): 1047-1048