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Resolution of human mercaptalbumin and nonmercaptalbumin by high performance liquid chromatography

, : Resolution of human mercaptalbumin and nonmercaptalbumin by high performance liquid chromatography. International Journal of Peptide & Protein Research 24(2): 96-103

Gel-exclusion high-performance liquid chromatographic (HPLC) analysis of human serum albumin (HSA) on PGP 2000 column (0.10 M sodium phosphate buffer, 0.30 M NaCl, pH 6.86) showed at least 2 peaks, the principal component corresponding to human mercaptalbumin (HMA) and the 2nd to human nonmercaptalbumin (HMA). Mechanism for the separation of HMA and HNA might be due to weak resin-HSA interaction. HPLC analysis of bovine plasma albumin (BPA) showed a single peak or PGP 2000 column. The elution volume of HSA was larger than that of BPA, resulting in a clear resolution of HSA and BPA.


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