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Resolution of human mercaptalbumin and nonmercaptalbumin by high performance liquid chromatography


, : Resolution of human mercaptalbumin and nonmercaptalbumin by high performance liquid chromatography. International Journal of Peptide & Protein Research 24(2): 96-103

Gel-exclusion high-performance liquid chromatographic (HPLC) analysis of human serum albumin (HSA) on PGP 2000 column (0.10 M sodium phosphate buffer, 0.30 M NaCl, pH 6.86) showed at least 2 peaks, the principal component corresponding to human mercaptalbumin (HMA) and the 2nd to human nonmercaptalbumin (HMA). Mechanism for the separation of HMA and HNA might be due to weak resin-HSA interaction. HPLC analysis of bovine plasma albumin (BPA) showed a single peak or PGP 2000 column. The elution volume of HSA was larger than that of BPA, resulting in a clear resolution of HSA and BPA.

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Other references

Renaud R.L.; Subden R.E., 1976: The molecular basis of antibiotic resistance in fungi. Canadian Journal of Genetics & Cytology 18(3): 573

Gillis, T.M.; Taylor, D.M.; Williams, D.R., 1998: Is hafnium a valid surrogate for plutonium for biochemical and biokinetic studies in vivo?. The MINTEQA2 computer code was used to simulate and compare the speciation profiles of hafnium (Hf4+) and plutonium (Pu4+) with citrate, oxalate, ethylenediaminetetraacetate (EDTA4-) and diethylenetriaminepentaacatate (DTPA5-) over the pH range 2-...

Martius, I., 1979: Local eccrine sweat gland hyperplasia. Dermatologische Monatschrift 165(5): 327-330

Magan, Ap, 1976: Regional centralization versus a centerless region. Planning Frontiers in Rural America Papers and Proceedings of the Boone Conference: ub 1976) 105-111

Jeyarani, S.; Rabindra, R.J.; Sathiah, N.; Karuppachamy, P.; Subramanian, S., 2007: Efficacy of spiracular infection of Helicoverpa armigera with its nucleopolyhedrovirus and its role in virus production. Baculoviruses are important microbial control agents of insects, with per os mode of infectivity. However, recently the spiracular infection of this virus group was suggested as an optimum method for virus production in grown up larvae. In this re...

Goldstein, H.E., 1975: Motivation as an approach to disease control. Journal of the American Veterinary Medical Association 167(9): 847-848

Biancifiori, C.; Bonser, G.M.; Caschera, F., 1962: Chemically induced mammary tumours following unilateral excision of the nipples in pseudopregnant and lactating breeding BALB/C mice. British Journal of Cancer 16: 232-237

Maiorana, M.; Ferri, D.; Convertini, G.; Montemurro, F.; Fornaro, F., 2005: Yield and quality of mixed winter fodder crops in Mediterranean environment. The aim of the two-year research is to evaluate, in temporary grasslands, forage mixtures able to ensure grazing during winter months and a good re-growth capacity after the grazing period and to study cutting times and to investigate the plant nu...

Krep, H.; Hossmann, K.A., 1999: Endothelin -antagonist improves neurologic outcome after cardiac arrest in rats. Anesthesiology (Hagerstown) 91(3A): A252, Sept

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