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Reversible inhibition of the in vitro coagulation of human plasma by lectins

, : Reversible inhibition of the in vitro coagulation of human plasma by lectins. Thrombosis and Haemostasis 48(2): 120-124

Wheat germ agglutinin (WGA) and concanavalin A (Con-A) (also red kidney bean agglutinin, PHA) have been found to be inhibitors of plasma clotting in vitro. At 40 micrograms/ml and 250 micrograms/ml (4.4 MicroM and 10 microM in carbohydrate binding sites, final concentrations) respectively, WGA and Con-A are able to double the activated partial thromboplastin time of normal human control plasma. Their inhibitory effect is due to their capacity to interact with the carbohydrate portion of blood clotting factors. It is totally abolished in the presence of specific saccharides for WGA or Con-A and is attenuated in the presence of 4% (v/v, final concentration) of human erythrocytes. The action of WGA is mediated by its ability to interact with N-acetylneuraminic acid. When purified phospholipid vesicles plus kaolin are used as an activator instead of cephalidin, this effect persists to the same extent. These two lectins also prolong the plasma clotting time using Russell's viper venom plus purified phospholipid vesicles as an activator. Quick's time was also prolonged by WGA and Con-A but to a lesser extent in this case. WGA can interact directly with some purified blood clotting factors (IX, X and II) in a classical lectin-glycoprotein precipitin reaction. When assessed at individual factors level in whole plasma using clogging assays, direct inhibitions by WGA are only apparent.


PMID: 6897462

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Campos, L.I.; Mansfield, D.; Smith, A.; Kohli, H.; Sun, D.; Espinosa, M.H.; Dy, V., 1995: Carbon dioxide volume and intra-abdominal pressure determination before the creation of a pneumoperitoneum. Laparoscopic surgery generally is regarded as a safe procedure when a preset pressure is used in the carbon dioxide insufflator. However, a fixed pressure setting is not appropriate when insufflating a very large or a very small abdomen. Presently...

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Lodin, H.; Ottander, H.G., 1966: Technical puncture complications in carotid angiography. The puncture complications in carotid angiography deserve more attention because of the wider and wider indications for this method of investigation. Routine radiographs should be taken of the injection area. Complications in the form of intramura...

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Smith, G.F.R., 1947: In pursuit of lethe: a short review of the progress of anaesthesia through the ages. Liverpool Medico-Chirurgical Journal 51(Pt 1): 5-46

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