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Rotenoid biosynthesis by tissue culture of derris elliptica


, : Rotenoid biosynthesis by tissue culture of derris elliptica. Agricultural & Biological Chemistry 44(10): 2387-2390

Callus induction was carried out by tissue culture using leaves of D. elliptica to obtain rotenoids. A small amount of rotenoids (2.9 .mu.g/g dry weight) was detected in the callus tissue thus induced and subcultured for 4 mo. Rotenoid biosynthesis decreased with frequent subcultures of callus tissue and was later lost. A callus with imperfectly differentiated rootlets was induced from the leaves or stems of derris by regulating plant hormones. This root-like organ contained rotenoids which were identified as rotenone and deguelin by gas chromatography-mass spectroscopy. The content was 160 .mu.g/g dry weight as rotenone.

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