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Secretin and cholecystokinin activities in extracts of sheep intestine

, : Secretin and cholecystokinin activities in extracts of sheep intestine. Veterinary Science Communications 4(1): 53-58

Extracts of sheep intestine prepared to contain secretin were readily obtained, reacted with antibodies raised to pure natural porcine secretin, were potent simultants of pancreatic exocrine secretion of the anesthetized rat and failed to contract sheep gall bladder in vitro. In contrast, extracts prepared to contain cholecystokinin (CCK) caused contraction of sheep gall bladder in vitro but were more difficult to obtain than those of secretin due to a contaminant green pigment. The experiments provide biological and immunological evidence for the existence of secretin in sheep intestine. The demonstration of CCK activity on sheep gall bladder by separate extracts is indicative of the presence of CCK in the sheep, but this awaits confirmation by immunological means.


DOI: 10.1007/bf02278480

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