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Secretory processes, carbohydrate and lipid metabolism in isolated mouse hepatocytes. Aspects of regulation by glucagon and insulin

, : Secretory processes, carbohydrate and lipid metabolism in isolated mouse hepatocytes. Aspects of regulation by glucagon and insulin. Biochimica et Biophysica Acta 428(2): 480-494

The procedure of Berry and Friend for isolation of intact hepatocytes has been adapted to mouse livers. The ultrastructure of these cells was satisfactorily preserved. Isolated mouse hepatocytes secreted proteins and triacylglycerols. These secretory processes were inhibited by colchicine, indicating a likely involvement of the microtubular system for their normal occurrence. Ultracentrifugation of medium incubated with hepatocytes, followed by electrophoresis and electron microscopic examination of the floating fraction (density less than 1.006) allowed to conclude that secreted triacylglycerols were very low density lipoproteins. Glycogenolysis and lipogenesis were stimulated or inhibited, respectively, by low concentrations of glucagon (10(-10) M). Other metabolic parameters were influenced by the hormone but were less sensitive to its action. Inhibition of lipogenesis by glucagon was associated with a decrease in acetyl CoA carboxylase activity. This decrease does not appear to be related to intracellular fatty acyl-CoA accumulation secondary to hepatic lipase activation by the hormone. Insulin was effective alone or counteracted glucagon effects on lipogenesis or glycogenolysis only when exposure of cells to collagenase was held minimal. This suggests that, during isolation of hepatocytes, insulin receptors may, for unknown reasons, be more fragile than those of glucagon.


PMID: 179577

Other references

Luther J.P., 1978: The inhibition of the lysis of aspergillus fumigatus by melanin a scanning electron microscopy study. Sturgess, J M (Ed ) Electron Microscopy , Vol Ii Biology Ninth International Congress Toronto, Ont , Canada, Aug 1-9, Xxxii+718p Illus Microscopical Society Of Canada: Toronto, Ont , Canada Isbn 0-920622-07-0 694

Durmaz, E.; Flanagan, S.; Berdeli, A.; Semiz, S.; Akcurin, S.; Ellard, S.; Bircan, I., 2013: Variability in the age at diagnosis of diabetes in two unrelated patients with a homozygous glucokinase gene mutation. Homozygous mutations in the glucokinase gene (GCK) result in a complete deficiency of the GCK enzyme, which leads to permanent neonatal diabetes mellitus. Whilst there has been one report of a patient (with a homozygous p.T168A) who was diagnosed...

Lee, K.H.; Imakura, Y.; Sims, D.; Mcphail, A.T.; Onan, K.D., 1976: Structure and stereochemistry of microlenin a novel anti tumor dimeric sesqui terpene lactone from helenium microcephalum x ray crystal structure. The structure and stereochemistry of microlenin, a novel antitumour sesquiterpene lactone isolated from H. microcephalum, were established from spectral and single-crystal X-ray analyses.

Fukushima, K.; Takahashi, K.; Fukushima, J.; Kato, M., 1987: Lack of suppression of the short-latency vestibulocollic reflex during active head movements in cats. It is a general belief that the vestibulocollic reflex (VCR) is suppressed when subjects make voluntary head movements, although clear evidence is still lacking. Experiments were performed to examine whether the short-latency VCR evoked by electri...

Prescott, GW.; Dillard, GE., 1979: A checklist of algal species reported from Montana, 1891 to 1977. PROCEEDINGS OF THE MONTANA ACADEMY OF SCIENCES: 38: 1-101 Monograph 1

Bagg, A., 2012: B cells behaving badly: a better basis to behold belligerence in B-cell lymphomas. A plethora of genetic abnormalities has been described in B-cell lymphomas, some of which arise when physiologic mechanisms involved in the generation of immunologic diversity go awry. Several different lymphoma types, such as follicular lymphoma...

Zar, H.J.; Lalloo, U.G., 2013: Acute asthma treatment guidelines: reducing morbidity and mortality in South Africa. South African Medical Journal 103(3): 159-160

Asselin, C.; Bastin, M., 1985: Sequences from polyomavirus and simian virus 40 large T genes capable of immortalizing primary rat embryo fibroblasts. We developed a procedure to evaluate quantitatively the capacity of subgenomic fragments from polyomavirus and simian virus 40 (SV40) to promote the establishment of primary cells in culture. The large T antigen from both of these viruses can immo...

Krohn, E.F., 1961: Epidemiological aspects of tuberculin sensitivity. Journal of Hygiene, Epidemiology, Microbiology, and Immunology 5: 161-167

Dolph G.E., 1985: Leaf form of the woody plants of indiana usa as related to environment. Margaris, N S , M Arianoustou-Faraggitaki And W C Oechel (Ed ) Tasks For Vegetation Science, Vol 13 Being Alive on Land; Proceedings Of The International Symposium on Adaptations to The Terrestrial Environment, Halkidiki, Greece, 1982 Ix+322p Dr W Junk Publishers: Hague, Netherlands; Boston, Mass , Usa (Dist By Kluwer Boston, Inc : Hingham, Mass , Usa; Kluwer Academic Publishers Group: Dordrecht, Netherlands) Illus 51-62, 1984