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Selective assimilation of selenite by Escherichia coli

, : Selective assimilation of selenite by Escherichia coli. Canadian Journal of Microbiology 28(3): 307-310

The assimilation of selenite by E. coli involves a transport process specific for this anion. Cystine, a repressor of sulfate and selenate uptake, when added to the growth medium, had no effect on selenite uptake; nor did sulfite inhibit assimilation of selenite. Cells grown aerobically in a basic salts medium transported selenite at an initial rate of 0.14 .mu.mol .cntdot. g-1 .cntdot. min-1 and a Vmax of 393.0 .mu.mol .cntdot. g-1 .cntdot. min-1. Cells grown in a medium that contained the trace elements necessary for synthesis of the selenoenzyme formate dehydrogenase took up selenite at a significantly faster rate (initial rate = 0.27 .mu.mol .cntdot. g-1 .cntdot. min-1, Vmax = 658.2 .mu.mol .cntdot. g-1 .cntdot. min-1). Km values for the transport process in the 2 media were similar. The results suggest the existence of a metabolic pathway, specific for selenite, responsible for the incorporation of Se into formate dehydrogenase.


PMID: 7044504

DOI: 10.1139/m82-045

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