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Selective cleavage of o acyl protecting groups from blocked deoxy ribotides


, : Selective cleavage of o acyl protecting groups from blocked deoxy ribotides. Bioorganic Chemistry 10(2): 161-168

Selective hydrolysis of the 3'-O-acetyl groups in the 5'-P-cyanoethyl-blocked deoxyribonucleotides, commonly used in oligomer synthesis by the diester method, can be achieved by mild hydrolysis with aqueous ammonia in pyridine for a brief period of time. The 3'-O-isobutyryl group is much more resistant. Conditions for 3'-unblocking of the 4 commonly used protected monomers as well as a model oligomer, d(CNEtphzA-ibuG)Ac, are described.

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