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Separation of non esterified chlorophylls by ion suppression high performance liquid chromatography


, : Separation of non esterified chlorophylls by ion suppression high performance liquid chromatography. Journal of Chromatography 298(1): 141-149

Chlorophyllides and pheophorbides [from Swiss chard leaves] were separated by ion-suppression high-performance liquid chromatography using an octadecyl silica column eluted with 80-95% (vol/vol) methanol in water containing 13 mM acetic acid (final pH 4.2) as the suppressing ion. The separated pigments were detected fluorometrically with a sensitivity in the picomol range without artifact formation. This technique can be used not only for the determination of non-esterified chlorophylls, but also for esterified chlorophylls, thus, enabling the simultaneous identification of chlorophylls and their derivatives.

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