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Solid phase elisa of insulin using beta lactamase from bacillus licheniformis 749 c and horseradish peroxidase as markers


, : Solid phase elisa of insulin using beta lactamase from bacillus licheniformis 749 c and horseradish peroxidase as markers. Antibiotiki i Meditsinskaya Biotekhnologiya 32(4): 279-282

Two modifications of solid phase enzyme immunoassay (EIAA) for insulin with the use of .beta.-lactamase from B. licheniformis 749/c and horseradish peroxidase as the markers were developed. .beta.-Lactamase and peroxidase conjugates with insulin were prepared with the glutaric aldehyde and periodate methods respectively. both the conjugates were stable and preserved high immunospecific and enzymatic activity. Sensitivity of EIAA with the use of the .beta.-lactamase or peroxidase as the markers was the same and amounted to 15 ng/ml of insulin. However, with the use of the .beta.-lactamase in EIAA not only spectrophotometric recording but also visual registration of the results was possible.

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