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Soluble chromogenic substrates for the assay of endo 1 4 beta xylanases ec and endo 1 4 beta glucanases ec

, : Soluble chromogenic substrates for the assay of endo 1 4 beta xylanases ec and endo 1 4 beta glucanases ec Analytical Biochemistry 144(1): 142-146

New soluble chromogenic substrates were prepared for specific and rapid assays of endo-1, 4-.beta.-xylanases and endo-1, 4-.beta.-glucanases. A soluble beechwood 4-O-methyl-D-glucurono-D-xylan was dyed with Remazol brilliant blue R and hydroxyethylcellulose was coupled to Ostazin brilliant red H-3B. The assays were based on photometric measurements of the enzyme-released dyed fragments soluble in the presence of organic solvents which precipitate the original substrates and their high-MW fractions. The assays are advantageous for rapid analyses of large amount of samples and also permit evaluation of the activities of both enzymes in the presence of exo-.beta.-glycanases and .beta.-glycosidases, at a high level of reducing compounds and viable cells, on the cell surface and on cell membranes and organelles.


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Sun, Y-Lan.; Huang, L-Qiao.; Pelosi, P.; Wang, C-Zhu., 2013: A lysine at the C-terminus of an odorant-binding protein is involved in binding aldehyde pheromone components in two Helicoverpa species. Odorant-binding proteins (OBPs) are soluble proteins, whose role in olfaction of insects is being recognized as more and more important. We have cloned, expressed and purified an OBP (HarmOBP7) from the antennae of the moth Helicoverpa armigera. W...

Finzi, G.F.; Camaggi, G.; Costa, C.; Stampi, S., 1979: Antibacterial activity of some disinfectants on bacterial strains isolated in a hospital environment. Experimental study. Archivio Per le Scienze Mediche 136(1): 83-98

Cuckle, H., 2010: Monitoring quality control of nuchal translucency. Nuchal translucency is the single most discriminatory marker for screening Down syndrome. When this marker is combined with concurrent maternal serum markers, the model-predicted performance is greater than for all second-trimester serum-only comb...

Scures, C.C., 1967: Report of the increase in bicanine diameter in 2 to 4-year-old children. Journal of Dentistry for Children 34(5): 332-335

Allen, A.R.; Doherty, R.; Hilton, A.M.; Freed, G.L., 2016: Inconsistencies in authoritative national paediatric workforce data sources. NlmCategory="UNASSIGNED"><b>Objective</b> National health workforce data are used in workforce projections, policy and planning. If data to measure the current effective clinical medical workforce are not consistent, accur...

Cogliati, M.; Meozzi, D., 1991: I posatoi del succiacapre (Caprimulgus europaeus). Atti Convegno Italiano di Ornitologia, 5: 27-30

Daza M.C.G., 1966: Clinico therapeutic investigation of phenanthroline quinone anti parasit in intestinal amoebiasis human entobex anti parasit. Med Rev Mex 46(1000): 540-544

Lee, B.P.; Dalsin, J.L.; Messersmith, P.B., 2001: Enzymatic and non-enzymatic pathways to formation of DOPA-modified PEG hydrogels. Abstracts of Papers American Chemical Society 222(1-2): POLY 424

Kambarami, R.A.; Matibe, P.; Pirie, D., 2000: Risk factors for neonatal mortality: Harare Central Hospital Neonatal Unit--Zimbabwe. To assess risk factors for neonatal mortality in a tertiary level neonatal unit. Case control analysis of routine neonatal data for 1998. Harare Central Hospital Neonatal Unit. All neonates delivered at Harare Maternity Hospital and admitted to th...

Goldfeder A.; Potmesil M.; Ghosh A.J.; Brown D., 1976: Cell kinetics of irradiated experimental tumors kinetic and structural characteristics of an in vitro transformed tumor. Radiation Research 67(3): 548