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Soluble chromogenic substrates for the assay of endo 1 4 beta xylanases ec 3.2.1.8 and endo 1 4 beta glucanases ec 3.2.1.4


, : Soluble chromogenic substrates for the assay of endo 1 4 beta xylanases ec 3.2.1.8 and endo 1 4 beta glucanases ec 3.2.1.4. Analytical Biochemistry 144(1): 142-146

New soluble chromogenic substrates were prepared for specific and rapid assays of endo-1, 4-.beta.-xylanases and endo-1, 4-.beta.-glucanases. A soluble beechwood 4-O-methyl-D-glucurono-D-xylan was dyed with Remazol brilliant blue R and hydroxyethylcellulose was coupled to Ostazin brilliant red H-3B. The assays were based on photometric measurements of the enzyme-released dyed fragments soluble in the presence of organic solvents which precipitate the original substrates and their high-MW fractions. The assays are advantageous for rapid analyses of large amount of samples and also permit evaluation of the activities of both enzymes in the presence of exo-.beta.-glycanases and .beta.-glycosidases, at a high level of reducing compounds and viable cells, on the cell surface and on cell membranes and organelles.

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