+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Studies of DNA polymerases alpha and beta from cultured human cells in various replicative states

, : Studies of DNA polymerases alpha and beta from cultured human cells in various replicative states. Journal of Cellular Physiology 126(1): 99-106

DNA polymerase activities from HeLa cells and from cultured diploid human fibroblasts in various growth states were compared. .alpha.-Polymerase activities from log phase fibroblasts treated with sodium butyrate and from stationary phase HeLa cells had DEAE-cellulose elution patterns that differed from those of polymerases from dividing cells. Moreover, .alpha.- and .beta.-polymerases from nondividing cells replicated synthetic polymers less faithfully. Although similar changes were observed previously for polymerases from late-passage and postconfluent early passage fibroblasts, amounts of .alpha.-polymerase activity recovered from nondividing cells in this study did not dramatically decline as they had in the former cases. The .alpha.-polymerase activities from HeLa cells and fibroblasts in various growth states sedimented near 7.5S in 0.4 M KCl and could be inhibited by a monoclonal IgG fraction prepared against KB cell .alpha.-polymerase. By several criteria, there was no significant differences in levels of UV-stimulated repair synthesis observed in early or late-passage postconfluent fibroblasts or in log phase fibroblasts treated with sodium butyrate. In summary, levels of .alpha.-polymerase do not necessarily correlate either with replicative activity or with apparent levels of repair synthesis. However, cells with decreased replicative activity always yielded enzyme with decreased fidelity in vitro and altered chromatographic behavior. It appears, therefore, that the alterations observed for .alpha.-polymerase from late-passage cells may be attributed more generally to the nondividing nature of these cells.


PMID: 3944201

DOI: 10.1002/jcp.1041260114

Other references

Waller, A.; Forshaw, K.; Bryant, J.; Mair, S., 2014: Interventions for preparing patients for chemotherapy and radiotherapy: a systematic review. Undergoing chemotherapy and radiotherapy can be physically and psychologically stressful for people with cancer. Providing preparatory information to cancer patients as they face treatment and its aftermath has the potential to improve patient out...

Ioachimescu, A.G.; Bauer, T.W.; Licata, A., 2008: Active crohn disease and hypercalcemia treated with infliximab: case report and literature review. To report a case of 1,25-dihydroxyvitamin D-mediated hypercalcemia caused by active Crohn disease that improved with infliximab therapy. We present the clinical and laboratory findings and describe the clinical course of a patient who had hypercal...

Rodriguez, A.A.; Rust, S.R.; Yokoyama, M.T.; Riquelme, E.O., 1995: Forage sorghum ensiled with silage additives at two stages of maturity under a tropical environment. Journal of Animal Science 73(SUPPL 1): 204

Chai, S.Y.; Vogelhut, P.O., 1965: Activation Energy of Direct-Current Electrical Conductivity of Ice with HF and NH3 Added. The activation energies of ice with varying amounts of impurities added were investigated with sintered platinum electrodes. For both types of impurities the activation energy was minimum at a concentration of 10(-4)M. The experimental arrangement...

Massard, G.; Eichler, F.; Gasser, B.; Bergerat, J.P.; Wihlm, J.M., 1998: Recurrence of the mediastinal growing teratoma syndrome. Annals of Thoracic Surgery 66(2): 605-606

Raymer J.H.; Velez G., 1990: The role of sfe in methods for the analysis of toxic compounds. U S Environmental Protection Agency's Atmospheric Research And Exposure Assessment Laboratory And Air And Waste Management Association Measurement Of Toxic And Related Air Pollutants; International Symposium, Raleigh, North Carolina, Usa, May 1-4, Xxi+1097p Air And Waste Management Association: Pittsburgh, Pennsylvania, Usa Illus Maps Paper : 69-75

Gencpinar, P.; Dundar, N.Olgac.; Tekgul, H., 2016: Electrical status epilepticus in sleep (ESES)/continuous spikes and waves during slow sleep (CSWS) syndrome in children: An electroclinical evaluation according to the EEG patterns. The aim of this study was to describe the electroclinical spectrum in children with electrical status epilepticus in sleep (ESES)/continuous spikes and waves during slow sleep (CSWS) syndrome according to the EEG patterns. Clinical data of 44 pati...

Andersen, N.D.; Nielsen, B.E.; Corradi, Jías.; Tolosa, Mía.F.; Feuerbach, D.; Arias, H.R.; Bouzat, C., 2016: Exploring the positive allosteric modulation of human α7 nicotinic receptors from a single-channel perspective. Enhancement of α7 nicotinic receptor (nAChR) function by positive allosteric modulators (PAMs) is a promising therapeutic strategy to improve cognitive deficits. PAMs have been classified only on the basis of their macroscopic effects as type I,...

Cho, S.K.m; Chae, H.B.ung; Boo,; Jin,; Lee, D.S.ung; Kim, D.W.on; Cho, M.J.e; Riu,, 2001: Isolation of genes involved in ecdysteroids biosynthesis from Achyranthes japonica Nakai. In order to isolate genes involved in ecdysteroids biosynthesis in plants, total RNA was isolated from Achyranthes japonica Nakai, and RT-PCR was performed using degenerate primers selected based on the results of multi-alignment of four cytochrom...

Boulé, J.B.; Rougeon, F.; Papanicolaou, C., 2001: Terminal deoxynucleotidyl transferase indiscriminately incorporates ribonucleotides and deoxyribonucleotides. Terminal deoxynucleotidyl transferase (TdT) catalyzes the condensation of deoxyribonucleotides on 3'-hydroxyl ends of DNA strands in a template-independent manner and adds N-regions to gene segment junctions during V(D)J recombination. Althou...