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Studies on alpha galactosidases purification and characterization of bacterial alpha galactosidase

, : Studies on alpha galactosidases purification and characterization of bacterial alpha galactosidase. Research Bulletin of Obihiro University Series I 9(3): 623-630

.alpha.-Galactosidase of Aerobacter sp. was purified about 110 fold by using column chromatography. This enzyme exhibited its absorption maximum at 211 nm showing little absorption at 280 nm. This enzyme, possessing a MW of 120,000, was extremely labile, and by means of pretreatment with 1 M NaCl lost its activity completely and irreversibly without a change of MW. In the presence of 6 M urea, there was complete loss of activity and the enzyme dissociated irreversibly into 2 subunits of different MW.


Other references

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Yoshimoto, Hiroshi, 2003: Appalachian tiger swallowtail, a most newly described papilionid. Pavulaan & Wright's (2002) work describing the Appalachian tiger swallowtail, Pterourus appalachiensis, is briefly introduced. A short history separating Pterourus canadensis is also viewed mainly based on Hagen et al.

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Smirnov Vvan V.Burt G.F.uerstein; Pellarin, M.; Marton, L.J.; Deen, D.F.; Basu, H.S., 1994: Pretreatment with the polyamine analog 1,19-bis- -5,10,15-triazanonadecane inhibits etoposide cytotoxicity in U-251 MG human brain tumor cells. We studied whether pretreatment of U-251 MG human brain tumor cells with the polyamine analog 1, 19-bis-(ethylamino)-5,10,15-triazanonadecane (BE-4-4-4-4) affected the cytotoxicity of the topoisomerase II inhibitor etoposide. We found that BE-4-4-...