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Studies on freezing injury in plants cells ii. protein and lipid changes in the plasma membranes of jerusalem artichoke helianthus tuberosus tubers during a lethal freezing in vivo


, : Studies on freezing injury in plants cells ii. protein and lipid changes in the plasma membranes of jerusalem artichoke helianthus tuberosus tubers during a lethal freezing in vivo. Plant Physiology (Rockville) 80(1): 187-195

Plasma membranes were isolated from both unfrozen and frozen tissues of Jerusalem artichoke tubers (Helianthus tuberosus L.) in high purity utilizing an aqueous two-polymer phase partition system. Although the recovery of the plasma membranes was decreased significantly by freezing of tissues even at the nonlethal temperature (-5.degree. C), the isolated plasma membrane samples were considered to be representative of the plasma membranes in situ. Freezing of the tissues at sublethal temperatures resulted in marked changes in the chemical composition of the plasma membrane. Those are losses of sterols and phosphatidylethanolamine from the plasma membranes, and a change of specific proteins with relatively high molecular weights into low molecular weight peptides. These specific proteins were designated as frost susceptible proteins. The properties of the plasma membrane ATPase seem to be not affected so much by the in vivo freezing of cells. However, inhibition of the plasma membrane ATPase by N,N'-dicyclohexylcarbodiimide (DCCD) was relatively low before and after freezing in vivo at the nonlethal temperature at -5.degree. C, but was markedly enhanced by freezing in vivo at sublethal temperatures below -10.degree. C. From the results, it is assumed either that the enzyme molecule was partially modified, especially at the presumed DCCD binding sites or that the DCCD had become more accessible to the enzyme as a result of increased permeability of the plasma membranes. These observed changes are discussed in connection with the mechanism of cell injury.

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Evans, J., 1976: Rodent problems in western forests. Proceedings of the Western Forestry Conference: 7th 60

Kurylcio, L.; Osemlak, J.; Obel, W., 1968: Left gastric artery in Macacus rhesus and Macacus cynomolgus. Folia Morphologica 27(2): 213-222

Dunning, A.J., 1985: Paroxysmal tachycardia and polyuria: a re-entry. Netherlands Journal of Medicine 28(10): 371-373

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Beutenmuller, W., 1894: Studies of some species of North American AEgeriidae. Bulletin of the American Museum, VI 87-98