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Substrate specificity of the milk clotting enzyme from irpex lacteus on peptide hormones


, : Substrate specificity of the milk clotting enzyme from irpex lacteus on peptide hormones. Agricultural & Biological Chemistry 50(4): 923-930

To clarify the substrate specificity of the milk-clotting enzyme excreted by Irpex lacteus (IR), various peptide hormones were used as substrates to find the cleavage sites and to compare the specificity with those of other milk-clotting enzymes such as calf chymosin (CR), Mucor miehei enzyme (MR), and Endothia parasitica enzyme (ER). IR hydrolyzed substance P, physalaemin, .beta.-neo-endorphin, neurotensin, angiotensin I, LHRH, and DSIP, but not bradykinin. Although IR generally require hydrophobic amino acids in the P1 and P1' sites, it also hydrolyzed peptide bonds which have only one hydrophobic amino acid either in the P1 or P1' site. CR did not hydrolyze either angiotensin I or DSIP, and MR did not hydrolyze DSIP, but both enzymes hydrolyzed the Tyr(5)-Glu(6)bond of LHRH slightly. On the other hand, ER hydrolyzed these substrates at several bonds. Besides the common cleavage sites with IR, ER hydrolyzed the His(6).sbd.Pro(7) bond of angiotesin I, the Leu(7).sbd.Arg(8) bond of LHRH, and the Ala(2).sbd.Gly(3) and Gly(3).sbd.Gly(4) bonds of DSIP. Therefore, these milk-clotting enzymes can be classified into two groups according to their substrate specificities on peptide hormones. CR and MR belong to the first group which has restricted specificity, while IR and ER belong to the second group which shows relatively broad specificity.

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Other references

Ladouceur, R., 2009: Sleep apnea and family physicians. Canadian Family Physician Medecin de Famille Canadien 55(1): 11, 13-11, 13

Feßler, A.T.; Calvo, N.; Gutiérrez, N.; Muñoz Bellido, J.Luis.; Fajardo, M.; Garduño, E.; Monecke, S.; Ehricht, R.; Kadlec, K.; Schwarz, S., 2014: Cfr-mediated linezolid resistance in methicillin-resistant Staphylococcus aureus and Staphylococcus haemolyticus associated with clinical infections in humans: two case reports. Journal of Antimicrobial ChemoTherapy 69(1): 268-270

Kaufman, Z.; Lively, J.; Carpenter, E., 1983: Uptake of nitrogenous nutrients by phytoplankton in a barrier island estuary: Great South Bay, New York. The uptake of urea, nitrate and ammonium by phytoplankton was measured using 15N isotopes over a one-year period in Great South Bay, a shallow coastal lagoon. The bay is a unique environment for the study of nutrient uptake since ambient concentra...

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Chari, C.R., 1959: Insecticidal trials of the control of early shoot borer, Chilotraea infuscatellus Snell, on sugarcane at Anakapalle. An increase of 9.8 tons per acre was recorded when the crop was sprayed with 0.32% DDT 4, 6 and 9 weeks after germination, the timing being based on the period of rapid tillering and oviposition by the borer.

Kerllenevich Sara Cecilia, 1988: Determination of Devonian population density for the Lolen Formation, Southern Ranges, Buenos Aires Province, using regression analysis. Actas - Jornadas Geologicas Bonaerenses 2: 87-97

Staffen, L.R., 1994: Heroic medicine, physician autonomy, and patient rights. Law & Social Inquiry 19: 753-773

Wan, L.; Thomas, Z.; Pisipati, S.; Jarvis, S.P.; Boutros, N.N., 2017: Inhibitory deficits in prepulse inhibition, sensory gating, and antisaccade eye movement in schizotypy. Schizotypy is a term that refers to a continuum of personality characteristics, emerging from mental states ranging from organized and normal to unorganized and disordered; with the latter tending to include individuals with high schizotypal score...

Garner, T.R.; Weinstein, J.E.; Sanger, D.M., 2008: Polycyclic aromatic hydrocarbon contamination in South Carolina salt marsh-tidal creek systems: relationships among sediments, biota, and watershed land use. Sediments and biota from 11 tidal creeks were sampled and classified into forested, suburban, and urban/industrial watershed land-use categories. Total PAH levels ( summation operatorPAH(16)) in sediments were significantly higher in urban/industr...

Muraguchi, A., 1986: Protein and polypeptide growth factors--B lymphocyte growth and differentiation factor. Nihon Rinsho. Japanese Journal of Clinical Medicine 44(1): 48-56