+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Superior sensitivity of conjugates of horseradish peroxidase with wheat germ agglutinin for studies of retrograde axonal transport

, : Superior sensitivity of conjugates of horseradish peroxidase with wheat germ agglutinin for studies of retrograde axonal transport. Journal of Histochemistry and Cytochemistry 27(3): 728-734

We have compared the retrograde axonal transport of horseradish peroxidase (HRP), to the retrograde transport of HRP conjugated with wheat germ agglutinin (WGA). Morphometric studies have shown that WGA-HRP conjugates were 40 times more sensitive than free HRP, in the tracing of retrograde connections from the rat submandibular gland to the superior cervical ganglion. Also, WGA-HRP was more sensitive than free HRP in the tracing of retrograde connections from the rat tongue to the hypoglossal nucleus. Our findings with WGA-HRP are consistent with the observations by Schwab et al. who reported (-125I) WGA is a highly sensitive retrograde tracer (Brain Research 152:145, 1978 (22)).


PMID: 90065

DOI: 10.1177/27.3.90065

Other references

Michel, J.F., 1969: The epidemiology of some nematode infections in calves. Helminthic disease occurs when susceptible animals acquire infection at an excessive rate. Heavy infestation on the pasture is derived either from some other group of animals or it may be built up by the same animals. The number of generations of...

Sebek, Z.S.xl, W.S.unzner, D.T.oger, H.C.armbouris, R., 1980: An investigation on the knowledge of blood parasites and parasitic tissue protozoa of free living small mammals from Greece. Folia parasitologicassued 27(3): 203-206

Haas, Jürgen.; Schwarz, A.; Korporal-Kuhnke, M.; Jarius, S.; Wildemann, B., 2016: Myeloid dendritic cells exhibit defects in activation and function in patients with multiple sclerosis. Regulatory T cells (Tregs) are functionally defective in patients with multiple sclerosis (MS) and this dysfunction is related to an imbalanced composition of naïve and memory Treg subtypes. Several lines of evidence indicate that these abnormali...

Dardeau G.; Pascal A., 1982: The terminal Jurassic regression in Alpes-Maritimes; stratigraphy, facies, sedimentary environment and influence of structural section in Nice Arc. Bulletin du Bureau de Recherches Geologiques et Minieres Section 1: Geologie de la France

van Dongen, J.J.; Quertermous, T.; Bartram, C.R.; Gold, D.P.; Wolvers-Tettero, I.L.; Comans-Bitter, W.M.; Hooijkaas, H.; Adriaansen, H.J.; de Klein, A.; Raghavachar, A., 1987: T cell receptor-CD3 complex during early T cell differentiation. Analysis of immature T cell acute lymphoblastic leukemias (T-ALL) at DNA, RNA, and cell membrane level. T cell acute lymphoblastic leukemias (T-ALL) can be regarded as the malignant counterparts of cells in various T cell differentiation stages. To study the expression of the human T cell receptor (TcR)-CD3 complex during the early stages of T cell...

Bauerle, C.; Ho, M.N.; Lindorfer, M.A.; Stevens, T.H., 1993: The Saccharomyces cerevisiae VMA6 gene encodes the 36-kDa subunit of the vacuolar H(+)-ATPase membrane sector. The yeast vacuolar membrane proton-translocating ATPase (V-ATPase) is a multisubunit complex comprised of peripheral catalytic, and integral membrane domains. At least eight proteins cofractionate with purified preparations of the enzyme including...

Salins, L.L.; Ware, R.A.; Ensor, C.M.; Daunert, S.; Daunert, S., 2001: A novel reagentless sensing system for measuring glucose based on the galactose/glucose-binding protein. The galactose/glucose-binding protein (GBP) is synthesized in the cytoplasm of Escherichia coli in a precursor form and exported into the periplasmic space upon cleavage of a 23-amino-acid leader sequence. GBP binds galactose and glucose in a high...

Okamoto, K.; Bereiter, D.F.; Tashiro, A.; Bereiter, D.A., 2009: Ocular surface-evoked Fos-like immunoreactivity is enhanced in trigeminal subnucleus caudalis by prior exposure to endotoxin. Endotoxin-induced uveitis (EIU) is a common animal model for anterior uveitis in humans that causes long-term changes in trigeminal brain stem neurons. This study used c-fos immunohistochemistry to assess the effects of different routes of adminis...

Ribeiro, F.; de Mendonça, A.; Guerreiro, M., 2006: Mild cognitive impairment: deficits in cognitive domains other than memory. Patients with mild cognitive impairment (MCI) typically present with memory complaints, but may have mild deficits in other cognitive domains. We compared the neuropsychological profiles of a series of consecutive MCI patients (n = 116) with a con...

Starup, J., 1971: Oral contraceptives more than 21 days?. Ugeskrift for Laeger 133(18): 887-887