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Surface characteristics of separated sub populations of mouse terato carcinoma cells

, : Surface characteristics of separated sub populations of mouse terato carcinoma cells. Experimental Cell Research 122(1): 39-45

Specific subpopulations of 129/J mouse teratocarcinoma cells were separated by velocity sedimentation in shallow density gradients using unit gravity or centrifugal force. The subpopulations were examined with perspective plots generated by a computer program. These plots permitted rotation of the horizontal axis making it possible to examine the symmetry of the major cell populations. The teratoma consistently separated into 2 major populations of cells, large cells and small cells. Although the small and large cells bound many of the 8 lectins tested, only the large cells agglutinated in the presence of low concentrations of lectins. The large cells are more malignant-like than the small cells with respect to their agglutinability characteristics. A heterogeneous, in vivo teratocarcinoma tumor apparently can be separated into specific cell populations that clearly differ with respect to certain cell surface characteristics.


PMID: 477757

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