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The 22 s cylinder particles of xenopus laevis 2. immunological characterization and localization of their proteins in tissues and cultured cells


, : The 22 s cylinder particles of xenopus laevis 2. immunological characterization and localization of their proteins in tissues and cultured cells. European Journal of Cell Biology 32(1): 157-163

Cylinder-shaped particles of 10 nm diameter were isolated from nuclei of Xenopus laevis oocytes and purified by sucrose gradient centrifugation and DEAE-Sephacel chromatography. Antibodies to protein constituents of these isolated particles were elicited in guinea pigs and examined by immunoblotting and immunoprecipitation techniques as well as by immunofluorescence microscopy. The antibodies reacted with only 2 out of the 12 constituent polypeptides characteristic for these particles when examined in the denatured state by the immunoblotting technique, including the largest component of MW 30,000, but were able to precipitate the whole ensemble of these polypeptides in immunoprecipitation experiments, in agreement with the notion that these proteins form the 22 S particle complex. The antibodies displayed a rather narrow range of interspecies cross-reactivity, showing reaction with cells of other amphibia but not with avian and mammalian cells. In oocytes as well as in transcriptionally active somatic cells the antigen was localized in the nucleoplasm, excluding nucleoli, as well as in the cytoplasm, usually suggesting a higher concentration in the nucleoplasm. During mitosis, the proteins were dispersed throughout the cytoplasm whereas the chromosomes were negative. Inactive cells such as mature erythrocytes, spermatids and spermatozoa were negative. These immunolocalization findings support the conclusion based on fractionation studies that the cylinder-shaped particles and their protein constituents occur both in the nucleoplasm and the cytoplasm of a broad range of cell types. The intracellular distribution of these frequent particles is discussed in relation to their size, which is at the upper limit for passage through nuclear pore complexes.

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PMID: 6667692


Other references

Navarrete, A.; Junco, M.L.; Gómez, M.A.; Beltrán, M.T., 1990: Late diagnosis of unilateral pulmonary hypoplasia. Report of a case. A 12 year old female with three to four annual episodes of respiratory tract infections since age 2 months, was admitted to the hospital with the chief complaint of acute bronchitis. During her hospital stay, the diagnosis of left pulmonary hypopl...

Osadchii, L.I.; Balueva, T.V.; Sergeev, I.V., 1993: Postural systemic vascular responses. Changes of hydraulic resistance of the vascular system were studied under conditions of stabilised blood expenditure in response to ortho- and antiorthostatic effects. The first type effects involved an increase in the resistance whereas the antio...

Sarkissian, Nér.; Maghari, A.; Rojas, J.; Lambert, W.Clark., 2011: Pseudomelanomas, pseudo-pseudomelanomas, and pseudometastasizing pseudomelanomas. Skinmed 8(5): 285-287

Lyalikova N.N., 1974: Stibiobacter senarmontii new species a new antimony oxidizing microorganism. Microbiology (New York) 43(6): (1975)

Mukhametdinova A.K.; Emtsev V.T., 1981: Transformation of various protein containing substrates by proteolytic anaerobes of the genus clostridium. In model experiments, transformation of substrate containing protein (native protein preparations, proteins of plant residues, as well as protein components of manure) by Clostridium proteolytic anaerobic bacteria was accompanied by formation of N...

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L.C.niat, M.; Romana, S.P.; Berger, R., 1995: Partial chromosome 21 amplification in a child with acute lymphoblastic leukemia. Monosomy 21 and metacentric markers corresponding in size to chromosomes 8 to 12 were found as the only clonal chromosomal changes in a child with acute lymphoblastic leukemia (ALL). Chromosome painting with a whole chromosome 21-specific probe sh...

Anonymous, 2013: Aldoxime dehydratase catalytic mechanism. Aldoxime dehydratase (OxdA), which is a unique heme protein, catalyzes the dehydration of an aldoxime to a nitrile even in the presence of water in the reaction mixture. Unlike the utilization of H2O2 or O2 as a mediator of catalysis by other heme...

Norris, M.L., 2016: Phosphate Supplementation During Refeeding of Hospitalized Adolescents With Anorexia Nervosa-Watch and Wait or Empirically Treat. Journal of Adolescent Health 58(6): 593-594