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The effect of halothane on the stability of calcium transport activity of isolated fragmented sarcoplasmic reticulum

, : The effect of halothane on the stability of calcium transport activity of isolated fragmented sarcoplasmic reticulum. Biochemical Pharmacology 29(3): 375-382

The effects of the inhalation anesthetic agent, halothane (CF3CHBrCl), on the stability of the Ca transport system of isolated rabbit white skeletal muscle sarcoplasmic reticulum were studied. Ca transport activity was unaffected when suspensions of sarcoplasmic reticulum vesicles were preincubated at C and pH 6.8 at concentrations of halothane below 5 mM, but was progressively inactivated at higher concentrations. (Ca2+, Mg2+)-ATPase [EC] activity was enhanced during inactivation of Ca transport. At pH 6.3 and 5.8, halothane increased the 1st order rate constants of inactivation and effects were noted in the anesthetic range of concentration (1-2 mM). The inulin inaccessible space of membrane vesicles did not change appreciably during the period of treatment with halothane, excluding increased permeability as an explanation of the inhibition of Ca accumulation. Inactivation was irreversible and highly temperature dependent, with an activation energy of 52.7 kcal/mol. Ca2+ had a protective effect against inactivation (K0.5[half-maximum inactivation constant](Ca2+) = 1.5 .times. 10-6 M), as did ATP (K0.5(ATP) .simeq. 10-6 M). Apparently mild acid conditions and halothane act synergistically during inactivation of the Ca transport system of sarcoplasmic reticulum membranes. Halothane may interact with the (Ca2+, Mg2+)-ATPase protein at the ATP-specific binding site or that it disrupts protein-lipid associations in the membrane. The destabilizing effect of halothane may be modified by the conformational state of the protein.


Other references

Savchenko, A.P.; Shendrik, I.G., 1976: X-ray anatomy of the inferior mesenteric artery according to findings of intravital angiography. The article describes the results of roentgenological analysis of 905 patients having no pathological manifestations in the basin of the inferior mesenteric artery. The percutaneous catheterization of the aorta after Seldinger was made in all the...

Tucker, Geoff, 1993: Pharmacology Drug delivery Lymphing along?. Lancet (North American Edition) 341(8856): 1314-1315

Komov Igor, 1996: Geochemical methods of prospecting for non-metallic minerals. International Geological Congres, Abstracts--Congres Geologique International, Resumes 30, Vol

Reed-Larsen, D.B.own, J., 1989: Bio-tech--study of parasitoid may lead to a new method of codling moth control. Proceedings Washington State Horticultural Association: 5th) 211

Sayed, R.; Cook, T.; Palmer, A., 2013: Recognizing isolated IgG4-related nephropathy. IgG4-related tubulointerstitial nephritis is an uncommon cause of renal impairment. It has been associated with dysfunction in a number of other organs giving rise to the term IgG4-related systemic disease; organ involvement can occur metachronous...

Hallman, G.J.; Wang, S.J.; Tang, J.M., 2005: Reaction orders for thermal mortality of third instars of Mexican fruit fly (Diptera: Tephritidae). Mexican fruit fly, Anastrepha ludens (Loew), is a quarantine pest of several fruit, including citrus, avocados, and mangoes, from extreme southern Texas to Costa Rica. To provide information for modeling heat phytosanitary treatments, third instar...

Nimmannit S., 1975: Purification and mechanism of action of yeast hydroxy methyl glutaryl coenzyme a reductase. Federation Proceedings: 567

Anonymous, 1967: Economic development issues: Latin America. One of the Praeger Special Studies in International Economics and Development, this book includes six papers studying key factors influencing the rates of growth in Latin America. Each paper is noted separately in this issue of WAERSA . D. K.

Ahmad, A.; Holloman, W.K.; Holliday, R., 1975: Nuclease that preferentially inactivates DNA containing mismatched bases. Nature 258(5530): 54-56

Merrell, R.W.; Brown, H.E.; Rose, J.F., 1979: Bladder carcinoma treated by partial cystectomy: a review of 54 cases. A retrospective study of 54 partial cystectomies done between 1958 and 1973 was undertaken to evaluate the proper role of the procedure in the treatment of bladder carcinoma. The operative mortality rate was 1.8% and the postoperative morbidity wa...