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The leakage of acrosomal hyaluronidase from the spermatozoa of the buffalo bubalus bubalis


, : The leakage of acrosomal hyaluronidase from the spermatozoa of the buffalo bubalus bubalis. Reproduction Nutrition Developpement 20(3 PART A): 593-599

The sonication method produced a quantitatively higher release of buffalo sperm hyaluronidase than the freeze-thaw technique. The released enzyme constituted 10% of the total enzymatic activity of fresh semen. Seminal plasma hyaluronidase activity was not correlated with the motility score of the semen sample. Unlike cattle semen, the seminal plasma enzyme level in buffalo semen stored at 37.degree. C showed a sharp rise; samples stored at 0.degree. C evidenced negligible enzyme leakage. Dilution of semen in citric acid whey at 5 or 37.degree. C significantly prevented the enzyme from leaking into the plasma although more enzyme was released in extended semen when it was exposed to cold treatments. The enzyme was stable in seminal plasma and acrosomal preparations when stored at 5.degree. C for prolonged periods.

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