+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Ultrastructural alterations in cells of hardened and nonhardened winter rye during hyper osmotic and extracellular freezing stresses

, : Ultrastructural alterations in cells of hardened and nonhardened winter rye during hyper osmotic and extracellular freezing stresses. Protoplasma 98(4): 329-342

Epicotyl tissues of cold-hardened and non-hardened winter rye (Secale cereale L. cv. Puma) were subjected to extracellular freezing ( C) and hyperosmotic stresses (1-4 M salt solutions) and were fixed such that the cellular plasmolytic and freezing patterns were retained to allow for examination of ultrastructural alterations during stress. Loss of integrity of the plasmalemma and the appearance of osmiophilic regions on the protoplasmic surface were observed and could be correlated with lethal cellular injury due to hyperosmotic and extracellular freezing stresses. Complete loss of ultrastructure of organelles such as etioplasts and mitochondria also occurred, but only at levels of hyperosmotic stress higher than that required to kill the cells. The formation of osmiophilic granules during hyperosmotic stress was irreversible since on deplasmolysis they were observed to outline the protoplasm at the point where it burst. Occasionally membranous connections between such osmiophilic granules could be observed after deplasmolysis. Similar osmiophilic granules were also observed on the outer membrane of some mitochondria. In the frozen-fixed cells, ultrastructural examination showed that the cell wall collapsed with the protoplast, indicating that the freeze pattern was retained by fixation. Osmiophilic regions on the protoplasmic surface and disruptions of the plasmalemma were also observed. Primary freezing injury to the cell occurs during the freezing process. Although the stresses between plasmolysis and freezing may be different in some respects, some of the ultrastructural manifestations of injury in both cases are similar.


Other references

Singh Pratap; Ramasatri K.S.; Kumar Naresh; Bhatnagar N.K., 2003: Suspended sediment transport from the Dokriani Glacier in the Garhwal Himalayas. Estimation of sediment load from glacierized basins is very important for planning, designing, installation and operation of hydro-power projects, including management of reservoirs. In the present study, an assessment of suspended sediment concen...

Zedgenidze G.A.; Amosov I.S., 1966: Blastomogenic effect of thorotrast on bones. Anon Trudy 8 Vsesoyuznogo S"ezda Rentgenologov I Radiologov, 1964 (Proceedings Of The 8th All-Union Congress Of Roentgenologists And Radiologists, 1964 ) Meditsina: Moscow, Ussr, From Ref Zh 1967, No 10 53 543-549

McSherry, T.J., 1987: Incompatibility between chlorpromazine and metacresol. American Journal of Hospital Pharmacy 44(7): 1574-1574

Wyrick, J.J.; Kyriss, M.N.M.; Davis, W.B., 2013: Ascending the nucleosome face: recognition and function of structured domains in the histone H2A-H2B dimer. Research over the past decade has greatly expanded our understanding of the nucleosome's role as a dynamic hub that is specifically recognized by many regulatory proteins involved in transcription, silencing, replication, repair, and chromoso...

Lankford, P.G., 1966: Rapid investigation of hemorrhagic emergencies. American Journal of Medical Technology 32(3): 155-158

Prasad, G.V.S.R., 1998: Effect of fumigation on viability and vigour of stored seeds of sorghum Moench cultivars. Studies to determine the effect of different frequencies of fumigation on sorghum seed stored under ambient conditions revealed that repeated fumigation for five times in the 10 months of storage had no deleterious effect on the viability and vigo...

Labavitch, J.; Rae, H., 1977: A purified polygalacturonase for cell wall analysis. Journal of food biochemistry: 1 (3) 301-309

Gegechkori, AM., 1983: Peculiarities in the biology of Psylloidea (Homoptera) from the Caucasus.. Vestnik Gosudarstvennogo Muzeya Gruzii, 32A 76-101

WangJingHong; WangKai; ZuoWenHui, 2008: Studies on cotton-rice rotation on the coastal area of Jiangsu Province. The cultivation pattern of paddy-upland rotation (using rice and cotton) was studied due to continuous cropping drawback in the coastal area of Jiangsu Province, China. Cotton-rice rotation not only improved the soil physical and chemical properti...

Warner, A.C., 1956: Criteria for establishing the validity of in vitro studies with rumen micro-organisms in so-called artificial rumen systems. An artificial rumen which attempts to reproduce as closely as possible conditions in the living animal is described. It consists of a cellophane dialysing sac, holding 50 ml. rumen fluid and substrate, suspended in a complex mineral solution based...