geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Uptake of estrone estradiol 17 beta and testosterone by isolated rat liver cells


, : Uptake of estrone estradiol 17 beta and testosterone by isolated rat liver cells. Biochemical and Biophysical Research Communications 77(2): 566-573

Estrone, estradiol-17.beta. and testosterone are taken up by isolated rat liver cells by a process which is saturable. The uptake was linear up to 10 s at 15.degree. C and up to 2 mg cell protein/ml. Under initial uptake conditions a 20-fold accumulation inside the cells was observed. Uptake of all 3 steroids is decreased by 2,4-dinitrophenol and antimycin A. The uptake of estrone and estradiol-17.beta. was inhibited by sodium ethylmercurithiosalicylate, iodoacetate, n-ethylmaleimide and 1-fluoro 2,4-dinitrobenzene; uptake of testosterone was not influenced by these reagents. .beta.-Glucosidase and .beta.-galactosidase inhibited the uptake of the 3 steroids by about 30%. Phospholipase A inhibited the uptake of estrone, estradiol-17.beta. and testosterone 39, 34 and 28%, respectively. Steroid hormones enter the cells by pathway(s) in which energy, proteins, sugars and lipids appear to be involved.

US$29.90

PMID: 901487


Other references

Sivanesan A., 1985: New species of bipolaris. Eight species of Bipolaris, B. coeffeana sp. nov., B. costina sp. nov., B. crotonis sp. nov., B. gossypina sp. nov., B. novae -zelandiae sp. nov., B. triticicola sp. nov., B tropicalis sp. nov. and B. zeae sp. nov., are described and illustrated.

Brecht, J.K.; Chau, K.V.; Fonseca, S.C.; Oliveira, F.A.R.; Silva, F.M.; Nunes, M.C.N.; Bender, R.J., 2003: Maintaining optimal atmosphere conditions for fruits and vegetables throughout the postharvest handling chain. Optimal controlled and modified atmospheres (CA and MA) for fresh produce vary according to the specie, its maturity or ripeness stage, the temperature, and the duration of exposure. However, individual lots of produce are typically handled for di...

Routtenberg, A.; Lindy, J., 1965: Effects of the availability of rewarding septal and hypothalamic stimulation on bar pressing for food under conditions of deprivation. Ten rats with electrodes aimed at medial forebrain bundle-posterior hypothalamus (MFB), and 4 rats with electrodes aimed at septal area were trained to press for food for 1 hr. per day. Food was available only during this session. After stable wei...

Britt, L.D., 2007: Simulation training: what are real questions that must be answered?. American Journal of Surgery 194(2): 220-220

May, P.A.; Fiorentino, D.; Coriale, G.; Kalberg, W.O.; Hoyme, H.Eugene.; Aragón, A.S.; Buckley, D.; Stellavato, C.; Gossage, J.Phillip.; Robinson, L.K.; Jones, K.Lyons.; Manning, M.; Ceccanti, M., 2011: Prevalence of children with severe fetal alcohol spectrum disorders in communities near Rome, Italy: new estimated rates are higher than previous estimates. To determine the population-based epidemiology of fetal alcohol syndrome (FAS) and other fetal alcohol spectrum disorders (FASD) in towns representative of the general population of central Italy. Slightly revised U.S. Institute of Medicine diagno...

Bashir, Y.; Ain, Q.Ul.; Jouda, M.; Al Sahaf, O., 2017: First Irish and tenth case of small bowel obstruction secondary to suprapubic catheterisation in the world. Case report and case review of a rare complication of suprapubic catheterisation. Percutaneous Suprapubic urinary bladder catheterisation (SPC) is a common procedure performed in cases of urinary retention where attempt to pass urethral catheter has failed. However, the procedure requires meticulous precision, vigilance and sou...

Nair, G.B.; Sarkar, R.K.; Chowdhury, S.; Pal, S.C., 1985: Campylobacter infection in domestic dogs. C. jejuni was recovered from the faeces of 10 (21.7%) of 46 domestic dogs with diarrhoea as compared with only one (3.1%) of 32 normal healthy dogs. The recovery rate from non-diarrhoeic, but unhealthy, domestic dogs was 6.7%. The differences in t...

Allan, D.; Michell, R.H., 1976: Production of 1,2-diacylglycerol in human erythrocyte 'ghosts' exposed to very low calcium ion concentrations. Biochemical Society Transactions 4(2): 252-253

Theuns H.M., 1981: Tooth morphology and caries. Nederlands Tijdschrift voor Tandheelkunde 88(7-8): 250-254

Jäkel, L.; Grün, E., 1977: Determination, proprerties and postnatal development of galactokinase in the swine liver. 1. Starting from the spectrophotometric method of Ballard optimal reaction conditions for measurements of galactokinase in piglet liver were systematically studied. These are (final conc. in the test): 100 mM triethanolamine-HCl buffer, 33 mM KCl,...