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Uptake of estrone estradiol 17 beta and testosterone by isolated rat liver cells

, : Uptake of estrone estradiol 17 beta and testosterone by isolated rat liver cells. Biochemical and Biophysical Research Communications 77(2): 566-573

Estrone, estradiol-17.beta. and testosterone are taken up by isolated rat liver cells by a process which is saturable. The uptake was linear up to 10 s at C and up to 2 mg cell protein/ml. Under initial uptake conditions a 20-fold accumulation inside the cells was observed. Uptake of all 3 steroids is decreased by 2,4-dinitrophenol and antimycin A. The uptake of estrone and estradiol-17.beta. was inhibited by sodium ethylmercurithiosalicylate, iodoacetate, n-ethylmaleimide and 1-fluoro 2,4-dinitrobenzene; uptake of testosterone was not influenced by these reagents. .beta.-Glucosidase and .beta.-galactosidase inhibited the uptake of the 3 steroids by about 30%. Phospholipase A inhibited the uptake of estrone, estradiol-17.beta. and testosterone 39, 34 and 28%, respectively. Steroid hormones enter the cells by pathway(s) in which energy, proteins, sugars and lipids appear to be involved.


PMID: 901487

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