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Use of a 2' 5' oligoadenylate analog probe for detecting rna ligase and rna ligase substrates in mammalian cell extracts


, : Use of a 2' 5' oligoadenylate analog probe for detecting rna ligase and rna ligase substrates in mammalian cell extracts. Biochemical and Biophysical Research Communications 123(1): 194-201

The compound ppp(A2'p)3A3'[32P]pCp is a commercially available radioactive analog of the 2',5' oligoadenylate series ppp(A2'p)nA, n .gtoreq. 2, commonly referred to as 2-5A. It is used as a probe for measuring concentrations in competition radiobinding and radioimmune assays. Incubation of the probe with extracts from HeLa, CV1 or neuroblastoma cells results in its covalent attachment to 2 size classes of RNA: the 1st includes a major species with a MW of approximately 350,000; the 2nd is much smaller (40 .+-. 5 nucleotides in length) and could represent tRNA half-molecules. Ligation is to the 3' end of the probe molecule with formation of a 3',5'-phosphodiester bond. Probe ligation provides a sensitive and convenient assay for the detection not only of RNA ligase(s) but also of ligatable RNA (such as the putative tRNA half-molecules) in mammalian cell extracts.

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Kim, E-A.; Park, J.; Kim, K-Hyung.; Lee, N.; Kim, D-Seong.; Kang, S-Kyu., 2012: Outbreak of sudden cardiac deaths in a tire manufacturing facility: can it be caused by nanoparticles?. The purpose of this study was to review clinical characteristics and working environments of sudden cardiac death (SCD) cases associated with a tire manufacturer in Korea, and review possible occupational risk factors for cardiovascular disease in...