+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Use of a 2' 5' oligoadenylate analog probe for detecting rna ligase and rna ligase substrates in mammalian cell extracts

, : Use of a 2' 5' oligoadenylate analog probe for detecting rna ligase and rna ligase substrates in mammalian cell extracts. Biochemical and Biophysical Research Communications 123(1): 194-201

The compound ppp(A2'p)3A3'[32P]pCp is a commercially available radioactive analog of the 2',5' oligoadenylate series ppp(A2'p)nA, n .gtoreq. 2, commonly referred to as 2-5A. It is used as a probe for measuring concentrations in competition radiobinding and radioimmune assays. Incubation of the probe with extracts from HeLa, CV1 or neuroblastoma cells results in its covalent attachment to 2 size classes of RNA: the 1st includes a major species with a MW of approximately 350,000; the 2nd is much smaller (40 .+-. 5 nucleotides in length) and could represent tRNA half-molecules. Ligation is to the 3' end of the probe molecule with formation of a 3',5'-phosphodiester bond. Probe ligation provides a sensitive and convenient assay for the detection not only of RNA ligase(s) but also of ligatable RNA (such as the putative tRNA half-molecules) in mammalian cell extracts.


Other references

Long, Yaotin, 1995: Study on the degradation of DNA in an acidic medium by HPLC using gradient elution. The determination and separation of bases from the degradation products of DNA in hydrochloric acid, perchloric acid, trichloroacetic acid, trifluoroacetic acid and formic acid were described. The hydrolysis yields of DNA adduct products of MNU al...

Balogh-Adam; Derzbach-Laszlo; Vasarhelyi-Barna, 2004: Hepcidin, a vashaztartas negativ iranyu regulatora. The task of the iron regulatory system is to balance between iron-stores and iron-consumption. Recently the predominant negative regulator of iron absorbtion in the small intestine and iron release from macrophages has been discovered. That is the...

Lombari G.; Lazarus D.B., 1988: Neogene cycladophorid radiolarians from north atlantic antarctic and north pacific deep sea sediments. This paper presents a detailed taxonomic analysis of cycladophorid radiolarians from Neogene high-latitude sediments of the Antarctic, North Pacific, and North Atlantic. The definition of the genus Cycladophora is emended to include many species p...

Cottier H., 1986: Qualitative and quantitative assessment of human polymorphonuclear leukocyte functions. Kretschmer, V. Stangel (ed.). Beitraege Zu Infusionstherapie Und Klinische Ernaehrung, Band 15. Transfusionsmedizin Aktuell-Infektionen, Thrombozyten, Granulozyten; Contributions To Infusion Therapy And Clinical Nutrition, (vol. 15. Transfusion Medicine Today: Infections, Thrombocytes, Granulocytes); Symposium Of The German Society For Blood Transfusion And Immune Hematology, Marburg, West Germany, Sept. 26-28, . Vii 293p. S. Karger Ag: Basel, Switzerland; New York, N.y., Usa. I

Amato R.V.; Furbush M.A.; Lees J.A., 1976: Potential geologic hazards related to drilling in the Atlantic outer continental shelf (OCS). Abstracts with Programs - Geological Society of America 8(2): 123-124

Iordanov, I.; Enchev, S.; Gromkov, N.; Vecelinova, A., 1974: Morphological changes in the skin of sheep and lambs after external application of Sr-90. Radiostrontium (as aqueous solution of the chloride) was applied to a 400 cm2 area of skin at the rate of 0.5 Ci/cm2 in twelve sheep. The sheep were killed for histological examination of the skin 6, 11 or 20-21 days afterwards. Proliferative, deg...

Munthe-Kaas, A.C., 1976: Phagocytosis in rat Kupffer cells in vitro. Experimental Cell Research 99(2): 319-327

Carranza-Lira, S.; Bueno Fontal, J.P., 2001: Modification of corporal weight, body fat distribution, blood lipids and glucose levels in oral contraceptive users. The association between oral contraceptives and the modification of corporal weight and body fat distribution is controversial. The characteristics of the menstrual cycle, lipids and glucose levels were also analyzed. Thirty women who received eth...

Prasil, J.S.vela, T.T.lecek, J., 1981: Participation of Veterinary Service in management of nutrition as a part of complex prevention of health disturbances in cattle. Veterinarstvi 1(11): 487-489

Kim, E-A.; Park, J.; Kim, K-Hyung.; Lee, N.; Kim, D-Seong.; Kang, S-Kyu., 2012: Outbreak of sudden cardiac deaths in a tire manufacturing facility: can it be caused by nanoparticles?. The purpose of this study was to review clinical characteristics and working environments of sudden cardiac death (SCD) cases associated with a tire manufacturer in Korea, and review possible occupational risk factors for cardiovascular disease in...