geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Use of positively selected lyt 2 plus mouse splenocytes to examine interleukin 2 secretion in responses to allo antigens and to tri nitro phenyl modified syngeneic cells


, : Use of positively selected lyt 2 plus mouse splenocytes to examine interleukin 2 secretion in responses to allo antigens and to tri nitro phenyl modified syngeneic cells. Cellular Immunology 68(1): 114-127

Current models for T cell interactions in the generation of cytotoxic T lymphocytes (CTL) have encountered a technical problem, since it has until recently been impossible to purify the peripheral Lyt-1+2+ subset from the Lyt-1+2- helper cell set. Reports that the helper factor Interleukin-2 (IL-2) can be synthesized by Lyt-2+ spleen cells have suggested that the peripheral Lyt-2+ set, unlike Lyt-2+ thymocytes, might not depend on help from Lyt-1+2- cells. To clarify this question, spleen Lyt-2+ cells and the complementary Lyt-2- set were produced by a positive selection method. The Lyt-2+ cells were able to produce high levels of anti-hapten CTL only if supplemented with either Lyt-2- cells or with semi-purified IL-2. Although IL-2 synthesis from Lyt-2+ cells, or from unseparated T cells, could be induced by H-2I region-disparate stimuli, Lyt-2+ cells produced very little IL-2 in response to H-2I or to H-2K region-disparate cells. IL-2 synthesis in hapten-stimulated cultures was found not to depend on the presence of the hapten per se, and probably represents a response to components of the fetal calf serum supplementation. Lyt-2+ cells were also much less able to generate IL-2 than Lyt-2- cells in response to these stimuli. Cell mixing experiments provided no evidence that Lyt-2+ cells could suppress IL-2 secretion by Lyt-2- cells. Generation of CTL from splenic Lyt-2+ cells apparently requires IL-2 produced by Lyt-2- cells, because Lyt-2+ cells do not produce high levels of IL-2 themselves, even when stimulated across an H-2K difference alone.

US$29.90


Other references

Riding Robert; Zhuravlev Andrey Yu, 1995: Structure and diversity of oldest sponge-microbe reefs; Lower Cambrian, Aldan River, Siberia. The oldest sponge reef is a small Early Cambrian bioherm at the base of the Tommotian Stage ( approximately 535-540 Ma) in southeast Siberia. The mainly archaeocyath construction may be a response to turbid conditions. Cambrocyathellus bowls fused...

Alimi, T., 1988: The prospect of large scale commercial dwarf goat production in South-Western Nigeria. Goat production in the humid tropics: proceedings of a workshop at the University of Ife Ile Ife Nigeria 20-24 July 1987 OB Smith and HG Bosman Eds: 158

Ekinci, F.J.; Shea, T.B., 1998: Selective activation by bryostatin-1 demonstrates unique roles for PKC epsilon in neurite extension and tau phosphorylation. Phorbol esters such as 12-O-tetradeonyl phorbol-13 acetate (TPA) induce a time-dependent biphasic effect on protein kinase C (PKC)-mediated events by fostering translocation of cytosolic (latent) PKC to the plasma membrane (where it is activated)....

Schoenwolf, G.C.; Garcia Martinez, V.; Darnell, D.K., 1996: Transfating of prospective mesoderm and neural plate in avian transplantation chimeras at late gastrula/early neurula stages. FASEB Journal 10(3): A804

Organ, C.H., 2000: On the nature of civility. Archives of Surgery 135(4): 395-395

Vikhrova, N.M.; Strukov, I.T.; Tebyakina, A.E.; Chaikovskaya, S.M.; Shneerson, A.N.; Dubova, V.G., 1965: Naphcillin and its microbiological study. Federation Proceedings. Translation Supplement; Selected Translations From Medical-Related Science 24(6): 1063-1066

Reed, M.D.; Rekate, H.L.; Aronoff, S.C.; Myers, C.M.; Blumer, J.L., 1983: Single-dose plasma and cerebrospinal fluid pharmacokinetics of ceftriaxone in infants and children. Pharmacokinetic variables were studied in children with central nervous system infections who received a single dose of ceftriaxone sodium. After initial lumbar puncture of children with documented or suspected bacterial meningitis, ventriculitis,...

Daniels, R.W.; Alcock, M.B.; Scarisbrick, D.H., 1982: A reappraisal of stem reserve contribution to grain yield in spring barley (Hordeum vulgare L.). Pre-anthesis stem reserve contribution to grain yield was assessed in spring barley cv. Mazurka (tall) and Goldfield (semidwarf). It was greatest in Mazurka but final grain yields were similar. Partitioning of total reserve capacity to various pla...

Bose, D.; Sarkar, A., 1973: Nodal and foliar vascularization in Mimosa pudica L. Science and culture: 39 (8) 358-361

Alajez, N.M., 2016: Significance of BMI1 and FSCN1 expression in colorectal cancer. Colorectal cancer (CRC) is the third most common type of cancer in terms of incidence and the fourth in cause of death world-wide, underscoring the need to identify novel biomarkers for early diagnosis, as well as improved disease stratification a...