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Zwitterionic detergent mediated interaction of purified cytochrome p 450l m 4 from 5 6 benzoflavone treated rabbits with nadph cytochrome p 450 reductase


, : Zwitterionic detergent mediated interaction of purified cytochrome p 450l m 4 from 5 6 benzoflavone treated rabbits with nadph cytochrome p 450 reductase. Biochemistry 26(8): 2343-2348

Hydroxylation of acetanilide catalyzed by purified cytochrome P-450Lm4 and NADPH-cytochrome P-450 reductase was reconstituted with the zwitterionic detergent 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS). The optimum rate of production of 4-hydroxyacetanilide was observed between 3 and 7 mM CHAPS and was about half that with 0.05 mM dilauroylglyceryl-3-phosphocholine (di-12-GPC). At higher detergent concentrations, hydroxylase activity decreased until at 15-20 mM CHAPS the system was inactive. The effect of CHAPS on the state of aggregation of P-450LM4 and on interaction between the cytochrome and P450 reductase alone and under turnover conditions was investigated by ultracentrifugation. At 4 mM CHAPS, P-450LM4 was hexameric to heptameric (Mr 369,000). Neither reductase nor reductase plus acetanilide and NADPH altered the state of P-450LM4 aggregation, suggesting that a stable 1:1 P-450/reductase complex did not form under turnover conditions. Replacing CHAPS with 0.05 mM di-12-GPC resulted in formation of heterogeneous P-450 oligomers (Mr > 480,000). At CHAPS concentrations where substrate hydroxylation did not occur (15 and 22 mM), P-450Lm4 was shown by sedimentation equilibrium measurements to be dimeric and monomeric, respectively. P-450 reductase was shown to reduce monomeric P-450LM4 in the presence of NADPH. This, the dependence of hydroxylase activity on [CHAPS] may be related to the state of aggregation of the cytochrome. An apprent correlation between P-450 aggregation state and NADPH-supported hydroxylation was also observed with phenobarbital-inducible P-450LM2 in the presence of detergents.

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PMID: 3113479


Other references

Krymskaya, V.P., 2007: Targeting the phosphatidylinositol 3-kinase pathway in airway smooth muscle: rationale and promise. The phosphatidylinositol 3-kinase (PI3K) signaling pathway plays a critical role in regulating cell growth, proliferation, survival, and motility. Structural alterations, e.g. airway remodeling, in asthma and chronic obstructive pulmonary disease...

Fidanza, J.; Glazer, M.; Mutnick, D.; McGall, G.; Frank, C., 2001: High capacity substrates as a platform for a DNA probe array genotyping assay. Colloidal silica particles were deposited on a glass substrate to produce high-capacity porous supports for high-density DNA probe arrays. Porous surfaces were used to increase the addressable surface area and number of probes available for hybrid...

Ercegovac Marko; Djaji Slavica; Krstic Branislav, 1995: Palynomorphs in marine Palaeozoic of the Carpatho-Balkanides, eastern Serbia. Special Publications of the Geological Society of Greece 4, Part 1: 181-185

da Silva, C.França.; Batista, D.da.Gama.Jaén.; de Araújo, J.Siciliano.; Cunha-Junior, Eézio.Ferreira.; Stephens, C.E.; Banerjee, M.; Farahat, A.A.; Akay, S.; Fisher, M.K.; Boykin, D.W.; Soeiro, M.de.Nazaré.Correia., 2017: Phenotypic evaluation and in silico ADMET properties of novel arylimidamides in acute mouse models of iTrypanosoma cruzi infection. Arylimidamides (AIAs), previously termed as reversed amidines, present a broad spectrum of activity against intracellular microorganisms. In the present study, three novel AIAs were evaluated in a mouse model of <i>Trypanosoma cruzi</i>...

Carls, P., 1969: Die Conodonten des tieferen Unter-Devons der Guadarrama (Mittel-Spanien) und die Stellung des Grenzbereiches Lochkovium/Pragium nach der rheinischen Gliederung. Senckenbergiana Lethaea, 50(4): 303-355

O'Reilly, M.K.; Tian, H.; Paulson, J.C., 2011: CD22 is a recycling receptor that can shuttle cargo between the cell surface and endosomal compartments of B cells. CD22 is a member of the sialic acid-binding Ig-like lectin (Siglec) family that is known to be a regulator of B cell signaling. Its B cell-specific expression makes it an attractive target for immunotoxin-mediated B cell depletion therapy for the...

Dietz, S.M.; Steenland, A.P.; Horner, C.E., 1951: Mint rust (Puccinia menthae) epiphytotic in the Northwest. An outbreak of mint rust (Puccinia menthae) caused 25 per cent. reduction in oil yield in Columbia County, Oregon, in 1949, and by the next year the disease had spread to every mint-growing area in the State. Six bi-weekly applications of 10 per c...

Waller, P.J.; Faedo, M., 1996: The prospects for biological control of the free-living stages of nematode parasites of livestock. Control of nematode parasites of livestock is focused almost entirely on the parasitic stages within the host. Current methods rely on anthelmintic drugs, but these are under increasing threat with the development of resistance covering the whole...

Wong, J.A.; Clark, J.F., 1968: Correlation of symptoms with age and location of gestation in tubal pregnancy. Journal of the National Medical Association 60(3): 221-223

Zigmantovich, I.M., 1985: Immediate and remote results of decompression of the celiac trunk. Results of elimination of the extravasal compression of the celiac trunk in 22 patients are analyzed. Unsatisfactory results of decompression can be explained by secondary alterations of the vessel due to the formation of scars. Thoracophrenolumbo...