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A statistical basis for using fewer rabbits in dermal irritation testing


, : A statistical basis for using fewer rabbits in dermal irritation testing. Journal of the American College of Toxicology 9(1): 49-60

An acceptable and validated in vitro method to evaluate the potential of a chemical to cause dermal irritation does not exist; therefore, in vivo studies remain the only alternative. Currently most laboratories utilize 6 rabbits per test, but this group size may not be necessary to derive the desired information. Data generated from 6-rabbit skin irritation tests of 105 materials were used to determine the ability of irritation scores from all possible combinations of 5-, 4-, 3-, or 2-rabbit subsets to predict the Draize score derived from 6 rabbits. There are 630, 1575, 2100, and 1575 possible combinations of 105 studies for the 5-, 4-, 3-, and 2-rabbit subsets, respectively. We classify materials using a four-level adjectival rating system based on (among other factors) the Draize score. Comparisons indicated that the 5-, 4-, 3-, and 2-rabbit scores were in 96, 94, 91, and 88% agreement, respectively, with the classification assigned on the basis of the 6-rabbit score. The correlation coefficients for randomly selected subsets of 5-, 4-, 3-, and 2-rabbit scores versus the 6-rabbit Draize score were 0.996, 0.994, 0.986, and 0.977, respectively. This study indicated that 3 rabbits per test group allow for adequate assessment of the dermal irritation potential of a chemical. These results also conformed closely to those obtained from a previous statistical study using eye irritancy data from 155 chemicals, where 3-rabbit subsets were 94% predictive of the 6-rabbit tests.

US$19.90

DOI: 10.3109/10915819009078717


Other references

Johnston J., 1985: Economic effects of an exotic disease outbreak in australia. Gibbs, A J And H R C Meischke (Ed ) Pests And Parasites As Migrants; Australian New Zealand Association For The Advancement Of Science Meeting, Canberra, A C T , Australia, May 15, 1984 Xiii+192p Cambridge University Press: Cambridge, England; New York, N Y , Usa Illus Maps Paper 58-62

Sorokin V.M.; Aliev A.A., 1974: Effect of fat supplements on the metabolism in lactating cows part 2 carbohydrate lipid metabolism in the liver of lactating cows after single administrations of saturated fatty acids. Byulleten' Vsesoyuznogo Nauchno-Issledovatel'skogo Instituta Fiziologii Biokhimii i Pitaniya Sel'skokhozyaistvennykh Zhivotnykh 4: 53-55 (FROM REF ZH ZHIVOTNOVOD VET BIOL OSN OTD VYP, 1975, 4

Garry P.J.; Vander Jagt D.J.; Wayne S.J.; Koehler K.H.; Rhyne R.L.; Simon T.L., 1991: A prospective study of blood donations in healthy elderly persons. Iron stores were observed in 57 healthy elderly volunteers, between 63 and 77 years of age, who donated 5 units of blood over approximately 1 year. An equal number of nondonors who contributed approximately 7 mL of blood at each visit for iron sta...

Gamboa, R.V.lozquez, J.P.desta, M., M., 1983: Trombiosis in cattle of Costa Rica. Ciencias veterinarias(2-3): 95-98

Gao Weihua; L.X.ng; Zhou Lin; E.A., 2002: Factors affecting the concentration of citrate solution in dialyzers when using citrate hemodialysate in vitro. Objective To observe the factors that affect the concentration of citrate solution in hollow fiber when using citrate hemodialysate. Methods By modeling hemodialysis in vitro, we used 6 types of hemodialyzers, different blood flow-rates, different...

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Balado, M.; Osorio, C.R.; Lemos, M.L., 2008: Biosynthetic and regulatory elements involved in the production of the siderophore vanchrobactin in Vibrio anguillarum. Some <i>Vibrio anguillarum</i> strains produce a catechol-type siderophore named vanchrobactin, whose biosynthetic pathway has not been completely elucidated. In addition to the previously described genes <i>vabA</i>, <i...

Fang, M.; Xi, S.; He, F., 1994: Nosocomial infections in gerontal patients in the Cadre ward. Nosocomial infections(NI) during hospitalization occurred in 149 cases, 9.8%. The NI rate was highest in the neurosurgery ward. Upper and lower respiratory tracts were the most frequent site of NI, followed by urinary tract and gastrointestinal tr...

Ikemoto, A.; Hirano, A., 1996: Immunohistochemical studies on clustered pericapillary bodies in the spinal cord of post-poliomyelitis patients. This report concerns the study of clustered pericapillary bodies in the spinal cords of four post-poliomyelitis patients. All four had stable neurological deficits without new neurological symptoms after the initial poliomyelitis infection. Spinal...

Corbett, S.L.; Frecker, H.M.; Shapiro, H.M.; Yudin, M.H., 2014: Access to fertility services for lesbian women in Canada. To determine reproductive services offered to lesbian patients by Canadian fertility clinics, policies of practice, ease of access to these services, and sensitivity of clinics to this population of patients. Survey sent to assisted reproductive t...