geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Activation of C3 and binding to Aspergillus fumigatus conidia and hyphae


, : Activation of C3 and binding to Aspergillus fumigatus conidia and hyphae. Infection and Immunity 57(11): 3412-3417

Complement activation by Aspergillus fumigatus may play a crucial role in stimulating binding and killing of this organism by phagocytes. We examined the amount and type of C3 deposited on resting conidia, swollen conidia, and hyphae of A. fumigatus after incubation in pooled human serum. All three life forms of A. fumigatus were potent activators of the complement cascade with deposition on the organisms of similar amounts of C3 per unit of surface area. The rate of deposition was slowest for resting conidia, although maximal deposition was still achieved within 40 min. The roles of the alternative and classical pathways were assessed by use of serum chelated with magnesium EGTA (magnesium ethylene glycol-bis(.beta.-aminoethyl ether)-N,N,N',N'-tetraacetic acid] and with an alternative pathway reconstituted from the six purified alternative-pathway proteins. Complement activation by resting conidia was mediated by the alternative pathway. In contrast, there was a progressive dependence on the classical pathway as the fungal particles matured into swollen conidia and then hyphae. Treatment with hydroxylamine, which disrupts ester linkages, removed 89 to 95% of the C3 bound to all three forms of A. fumigatus. This released C3 contained a mixture of C3b and iC3b, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography. These data demonstrate that although all three forms of A. fumigatus are potent activators of the complement system, the transition from resting conidia to swollen conidia to hyphae results in progressive changes in the manner in which the fungal particles interact with the complement system. The lack of participation of the classical pathway in complement activation by resting conidia may hve important implications regarding their ability to effectively stimulate phagocytes.

US$29.90

PMID: 2680973


Other references

Gao, Q.; Meguro, H.; Okamoto, S.; Kimura, M., 2013: Flexible tactile sensor using the reversible deformation of poly(3-hexylthiophene) nanofiber assemblies. In this letter, we report a simple approach to fabricating scalable flexible tactile sensors using a nanofiber assembly of regioregular poly(3-hexylthiophene) (P3HT). Uniform P3HT nanofibers are obtained through a continuous electrospinning proces...

Goodberlet, M.A.; Swift, C.T.; Kiley, K.P.; Miller, J.L.; Zaitzeff, J.B., 1997: Microwave remote sensing of coastal zone salinity. An operational airborne salinity remote sensing system is now available for coastal and estuarine studies with an accuracy of 1 ppt at 100 m spatial resolution. The system can perform area mapping at a rate of 100 km-2/hr from low altitude light a...

Dzialo, M.C.; Travaglini, K.J.; Shen, S.; Roy, K.; Chanfreau, G.F.; Loo, J.A.; Clarke, S.G., 2015: Translational roles of elongation factor 2 protein lysine methylation. Methylation of various components of the translational machinery has been shown to globally affect protein synthesis. Little is currently known about the role of lysine methylation on elongation factors. Here we show that in Saccharomyces cerevisi...

Peltzer, M.A.; Schardein, J.L., 1966: A convenient method for processing fetuses for skeletal staining. Stain Technology 41(5): 300-302

Lacazedieu G.; Vouve J., 1977: Difficulties in the definition of protection perimeter outlines linked to the frequency of geometric distortions between the zone of influence and the area of recharge of water catchments. Pages 125-136 1977

Ricksten, S-Erik., 2006: Errors in table, not "diametrically opposite result"!. Critical Care Medicine 34(6): 1859-1859

Iwasaki T.; Yoshinaga S.; Hirakawa K., 1991: River terraces along the Motoura and Hidaka-Horobetsu rivers in the southern Hidaka Range, Hokkaido. Chirigaku Hyoron = Geographical Review of Japan Pages 597-612

Rosenberg, G.H.; Alahari, S.K.; Käufer, N.F., 1991: prp4 from Schizosaccharomyces pombe, a mutant deficient in pre-mRNA splicing isolated using genes containing artificial introns. We have generated a bank of temperature-sensitive (ts) Schizosaccharomyces pombe mutant strains. About 150 of these mutants were transformed with a ura4 gene containing an artificial intron. We screened these ts mutants for mutants deficient in sp...

Tsai, F.C.; Gardner, D.K., 1994: Nicotinamide, a component of complex culture media, inhibits mouse embryo development in vitro and reduces subsequent developmental potential after transfer. Objective: To determine the effects of B-group vitamins present in culture media on mouse embryo development in vitro and subsequent viability. Design: Mouse zygotes were cultured in the presence of B-group vitamins. Embryo morphology and cell num...

Huang, T.X.; Ding, Y.K.; Zheng, Z.J.; Miao, W.Y.; Cao, Z.R.; Jiang, S.E.; Liu, S.Y.; Liu, Z.L., 2008: Unfolding core asymmetries with x-ray emission images in symmetry diagnostic experiments. A novel inversion technique is proposed to unfold core asymmetries at the source with x-ray emission images, which were obtained from imploded surrogate capsules in symmetry diagnostic experiments. The axisymmetrical core emission can be expanded...