geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Addition of extra origins of replication to a minichromosome suppresses its mitotic loss in cdc6 and cdc14 mutants of Saccharomyces cerevisiae


, : Addition of extra origins of replication to a minichromosome suppresses its mitotic loss in cdc6 and cdc14 mutants of Saccharomyces cerevisiae. Proceedings of the National Academy of Sciences of the United States of America 89(7): 3098-3102

Many cell division cycle (cdc) mutants of Saccharomyces cerevisiae exhibit elevated mitotic loss of pDK243, a 14-kilobase minichromosome with a centromere and one autonomous replicating sequence (ARS). Tandem copies of different ARSs were added to pDK243. The addition of these ARS clusters to pDK243 had no effect on its mitotic loss in cdc7 (protein kinase), cdc9 (DNA ligase), or cdc16 or cdc17 (DNA polymerase) mutants. However, in cdc6 and cdc14 mutants, the mitotic loss of pDK243 with an ARS cluster was suppressed by a factor of 6-8 compared to pDK243 without the cluster. This suppression was dependent upon the number of ARSs in the cluster and the integrity of the ARS consensus sequence in each ARS of the cluster. ARSs are known to be DNA replication origins. Therefore, the suppression of mini-chromosome loss by ARSs in cdc6 and cdc14 mutants suggests that these mutants are defective in the initiation of DNA replication. Since the CDC6 protein appears to act at the G1/S phase transition, the CDC6 protein may be a factor required at the beginning of S phase to initiate DNA replication at origins. In contrast, the CDC14 protein acts after mitosis. We suggest that the CDC14 protein performs a function late in the cell cycle that may be required for efficient initiation of DNA replication during S phase of the next cell cycle.

US$19.90

PMID: 1557417

DOI: 10.2307/2359041


Other references

Tilse, M.H.; McAlister, T.V., 1981: Isolation of Campylobacter fetus from blood cultures. Campylobacter fetus subsp. fetus, previously known as Campylobacter fetus subsp. intestinalis, was isolated from two blood cultures taken from a 45-year-old man admitted with gastrointestinal symptoms and a history of lymphocytic lymphoma. Campylo...

Voigt, K.; Brügmann, M.; Huber, K.; Dewar, P.; Cousens, C.; Hall, M.; Sharp, J.M.; Ganter, M., 2007: PCR examination of bronchoalveolar lavage samples is a useful tool in pre-clinical diagnosis of ovine pulmonary adenocarcinoma (Jaagsiekte). Ovine pulmonary adenocarcinoma (OPA) is a contagious lung tumour of sheep caused by Jaagsiekte sheep retrovirus (JSRV). The disease is a particular problem in flocks in many parts of the world. The aim of the study was to assess screening methods...

Whelan, B.R.; Barrow, N.J., 1984: The movement of septic tank effluent through sandy soils near Perth. I. Movement of nitrogen. The movement and transformation of nitrogen under seven septic tank installations was studied. Samples of the effluent and of the soil solution beneath the discharge systems were taken and analysed for ammonium, nitrate and pH. Most of the nitroge...

Feksi A.T.; Kaamugisha J.; Sander J.W.A.S.; Gatiti S.; Shorvon S.D., 1991: Comprehensive primary health care antiepileptic drug treatment program in rural and semi urban kenya. A programme of antiepileptic treatment in a rural and semi-urban region in Kenya was assessed. Patients with generalised tonic-clonic seizures were treated according to one of two simple drug protocols. Health workers screened cases reported by ke...

Zuckerberg, H.D.; Snow, G.R., 1968: What do parents expect from the physician? A resume of recent opinions. Pediatric Clinics of North America 15(4): 861-870

Besheer, J.; Bevins, R.A., 2003: Impact of nicotine withdrawal on novelty reward and related behaviors. The authors tested the decreased reward function hypothesis of nicotine withdrawal using a novel-object place conditioning task. A conditioned place preference was evident in controls and in rats that had experienced 4 nicotine withdrawal days, bu...

Christakos S.; Varghese S.; Huang Y C., 1988: Renal calcium binding protein distribution and regulation of expression. AAAS (American Association for the Advancement of Science) Publication (88-30): 68

Koshland, D.E., 1991: Response. The author responds to comments made by F. Ruttner [Journal of Apicultural Research (1991) 30 (2) 113-114] concerning his [Moritz's] assessment of attempts by German bee breeders to replace the Apis mellifera mellifera population with A. m. c...

D.T.mbe, P.P.; Wannenburg, T.; Fan, D.; Little, W.C., 1996: Right ventricular contractile protein function in rats with left ventricular myocardial infarction. We studied contractile function in cardiac trabeculae isolated from the right ventricles (RV) of rats with experimental heart failure (HF) induced by left ventricular (IV) myocardial infarction (24 wk post-MI; n = 6) and from sham-operated rats (n...

Raghu, C.N.; Manikeri, S.R.; Sheth, U.K.; Dadkar, V.N., 1982: Probable mode of taurine action. Taurine-Ca relationship was studied at the cellular level using [human] platelets as a test model system. Taurine at pH 7.2 produced a dose-related inhibition of aggregation induced by ADP, epinephrine and collagen. Ca in doses insufficient to alt...