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Arachidonate supports hydrolysis of phosphatidylinositol by neutrophil cytosolic phospholipase C: relation to NADPH oxidase


, : Arachidonate supports hydrolysis of phosphatidylinositol by neutrophil cytosolic phospholipase C: relation to NADPH oxidase. Biochemistry International 23(4): 751-758

NADPH oxidase is a superoxide-generating, membrane-bound complex activated in stimulated phagocytes or in a reconstituted system consisting of membranes, cytosolic components and arachidonate or SDS. To delineate mechanism of oxidase activation in the cell-free system, hydrolysis of phosphoinositides in the combined membrane-cytosol oxidase mixture was investigated. Arachidonate promoted hydrolysis of membrane-[3H]-phosphatidylinositol by cytosolic phospholipase C. PI hydrolysis was similarly supported by other unsaturated fatty acids and by SDS. Unlike activation of the NADPH oxidase, PI hydrolysis required the presence of calcium ions. Implications of these findings to the mechanism of NADPH oxidsae activation are discussed.

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PMID: 1908242


Other references

Schwartz, B.F.; Stoller, M.L., 1999: Complications of retrograde balloon cautery endopyelotomy. Purpose: Adult ureteropelvic junction obstruction is increasingly managed with endoscopic techniques. Retrograde balloon cautery endopyelotomy is quick, requires minimal hospital stay and allows most patients a rapid return to work. The complicati...

Langmann, R., 1966: Early recognition of diabetes and control of accompanying diseases. Possibilities of preventive health care for diabetics. Der Offentliche Gesundheitsdienst 28(6): 222-226

Kim H.W.; Brandt C.D.; Arrobio J.O.; Murphy B.; Chanock R.M.; Parrott R.H., 1979: Influenza a and influenza b virus infection in infants and young children during the years 1957 1976. Influenza A virus activity was demonstrated in infants and young children from metropolitan Washington, D.C. [USA] during each of 19 successive Aug.-July respiratory disease years, and during 17 of these years at least 2% of hospitalized respirato...

Gabriel Henrique Horta de Oliveira; Paulo Cesar Corrêa; Emílio de Souza Santos; Pedro Casanova Treto; Mayra Darliane Martins Silva Diniz, 2011: Evaluation of thermodynamic properties using GAB model to describe the desorption process of cocoa beans. The desorption isotherms and thermodynamic properties of cocoa beans were obtained during the drying process of this product. The isotherms were determined by dynamic method for various temperature (25, 35, 45 and 55 C) and relative humidity (RH)...

Sawaya M.E.; Mendez A.J.; Hsia S.L., 1988: Translocation of androgen receptor protein complex into nuclei of hair follicles and sebaceous glands from human scalp. Clinical Research 36(3): 692A

Balaraman, K.; Hoti, S.L., 1988: Comparative costs of mosquito control with larvicidal bacilli and insecticides. The production costs of the microbial pesticides Bacillus thuringiensis H14 [B. thuringiensis subsp. israelensis] and B. sphaericus were worked out in India based on the input used to produce them in a 13-litre laboratory fermentor. The production...

Gorski, R.A., 1973: Perinatal effects of sex steroids on brain development and function. Progress in Brain Research 39: 149-163

Bajusz, E.; Selye, H., 1959: Effect of various hormones upon the muscular contractions induced by sodium perchlorate. In rats peroral or subcutaneous administration of NaCl4 produces characteristic contractions of striped muscles. Parathyroidectomy and daily administration of mineral corticoids (2a-methyl-9a-chlorocor-tisol, 100 ug per dose, desoxycorticosterone...

Gayatri, M.C.; Kavyashree, R., 2007: Effect of carbon sources on in vitro seed germination of monopodial orchid - Epidendrum radicans. The effect of sorbitol, sucrose, glucose, fructose, maltose, lactose and galactose carbon sources at various concentrations on in vitro seed germination and protocorm formation in Epidendrum radicans has been studied. The seeds were cultured on K...

D.E.izalde D.B.acco M.M.; Riera N.; Galassi N.; D.L.B.rrera S.; Anselmo A.; Perez Bianco R., 1992: Autoantibodies in hemophilia are there consequences of hiv i infection and or cofactors in the development of the infection?. Sangre (Saragossa) 37(3): 225-236