geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Characterization of the relation between sodium channels and electrical activity in cultured rat skeletal myotubes regulatory aspects


, : Characterization of the relation between sodium channels and electrical activity in cultured rat skeletal myotubes regulatory aspects. Brain Research 488(1-2): 186-194

The relation among sodium channel density, frequency of electrical activity and maximal rate of rise of the action potential was studied in developing and mature rat skeletal myotubes in culture. The number of tetrodotoxin (TTX)-sensitive Na-channels was determined by measurements of the amount of [3H]saxitoxin (STX) bound to the cultures, and electrical properties were recorded with intracellular microelectrodes. The EC50 for TTX-induced decreases in maximal STX-binding, frequency and rate of rise of action potentials was in the range 8-20 nM. The 3 variables increased in parallel with age in culture to reach peak values at age 7-8 days, and then decreased in parallel until 10-20 days in culture. The age-related increase in Na-channel density was decreased, but not abolished, by prevention of myoblast fusion. Treatment with the Ca2+ ionophore, A23187, down-regulated, and blockade of Ca-channels with verapamil up-regulated the number of Na-channels. Na-channel density was also increased by chronic treatment with TTX and elevated external [K+], which eliminated spontaneous electrical and contractile activity. Parallel effects were observed on frequency and rate of rise of action potentials. Up-regulation of Na-channels was prevented by simultaneous treatment of myotubes with inhibitors of protein synthesis. We conclude that electrical and mechanical activity of cultured myotubes regulate de nove synthesis of Na-channels through alterations in the level of cytosolic Ca2+.

US$19.90

PMID: 2545301

DOI: 10.1016/0006-8993(89)90708-7


Other references

Liao, Q.; Guo, X.; Li, X.; Li, X.; Chen, P.; Liang, F.; Tang, H.; Deng, M.; Wu, M.; Ma, J.; Xiong, W.; Li, G., 2012: Analysis of the contribution of nasopharyngeal epithelial cancer cells to the induction of a local inflammatory response. Journal of Cancer Research and Clinical Oncology 138(1): 57-64

Harris, P.; Schaare, P.; Cook, C.; Henderson, J., 2001: An ambulatory physiological monitor for animal welfare studies. Monitoring physiological activity in freely moving subjects poses a considerable challenge. The Free-Range Physiological Monitor (FRPM) is a novel instrumentation pack designed to record and analyze physiological processes in ambulatory subjects f...

Dawson, S., 1998: Pre-amputation assessment using Roy's Adaptation Model. This article describes the use of Roy's Adaptation Model as a framework for the assessment of a 69-year-old man undergoing a right below-knee amputation. The model recognizes that individuals are subject to internal and external stressors whi...

Al-Jarallah, A.S.; Lardhi, A.A.; Hassan, A.A., 2004: Endocarditis prophylaxis in children with congenital heart disease. A parent's awareness. Objectives: The aim of this study is to determine the parental knowledge of bacterial endocarditis prophylaxis (BEP). Methods: Parents of 205 patients attending the Pediatric Cardiology Clinics at King Khalid University Hospital, Riyadh, King Faha...

Lee, C-Ki.; Bernhard, B.Jason.; Kim, J.; Fong, T.; Lee, T.Kyung., 2016: Differential Gambling Motivations and Recreational Activity Preferences Among Casino Gamblers. This study investigated three different types of gamblers (recreational, problem, and pathological gamblers) to determine differences in gambling motivations and recreational activity preferences among casino gamblers. We collected data from 600 g...

Goodnough, Lawrence T., 2002: Transfusion medicine Dr Goodnough replies. New England Journal of Medicine 347(7): 538-539, August 15

Braillon P.; Duboeuf F.; Delmas P.D.; Meunier P.J., 1987: Dual photon absorptiometry measurement of the lumbar bone mineral contents methodology reproducibility and normal values. Measurements were made with a DPA apparatus (Novo Lab 22a) on different phantoms and on volunteers in an attempt to evaluate the system precision. The reproductibility was found in the range of 0.98 to 4.10% in the case of in vitro measurements, d...

Sakai, R.; Irie, Y.; Murata, T.; Ishige, A.; Anjiki, N.; Watanabe, K., 2007: Toki-to protects dopaminergic neurons in the substantia nigra from neurotoxicity of MPTP in mice. Parkinson's disease (PD) is a neurodegenerative disease of the brain characterized by the progressive loss of dopaminergic neurons in the substantia nigra (SN). No clinically proven drugs that may halt or retard the progression of PD have be...

Grattan-Smith, M., 1971: Caesarean section follow-up survey--New South Wales, 1968-1969. Medical Journal of Australia 1(16): 856-857

Bush, T.; Levine, M.D.; Zbikowski, S.; Deprey, M.; Rabius, V.; McAfee, T.; Wiatrek, D.E., 2008: Weight Gain After Quitting: Attitudes, Beliefs and Counselling Strategies of Cessation Counsellors. Postcessation weight gain is common and a frequent cause of relapse. Although interventions to address weight gain and weight gain concerns exist, the experience of telephone cessation coun- sellors in addressing weight concerns is unknown. We sur...