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Chemical reactivity of matched cytosine and thymine bases near mismatched and unmatched bases in a heteroduplex between DNA strands with multiple differences


, : Chemical reactivity of matched cytosine and thymine bases near mismatched and unmatched bases in a heteroduplex between DNA strands with multiple differences. Nucleic Acids Research 17(11): 4223-4233

The chemical reactivity of matched T and C bases to osmium tetroxide and hydroxylamine near mismatched and unmatched bases in a heteroduplex between two strands of DNA with multiple differences was examined. Data was available for matched bases one or two positions away from 24 mismatches. Reactive bases were found near 16 of the mismatches and were usually one or two bases away. This reactivity is consistent with structural studies indicating perturbation of the duplex around mismatches and will allow another mode of study of the effect of mismatches. The reactivity of these bases was found not to be strongly correlated with mismatch type or GC basepair content of the basepairs around the mismatches. Extra reactivity may have been promoted by the presence of either T or C in the mismatch allowing increased reactivity of nearby T or C. The utility of the phenomenon for the detection of mutations is discussed. Unmatched bases in the heteroduplex also gives rise to reactive matched bases nearby.

US$19.90

PMID: 2787020

DOI: 10.1093/nar/17.11.4223


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Easton, ER.; Lysyk, TJ., 1986: Response of a laboratory strain of the face fly to seasonal changes in manure quality. Pupal weight and head-capsule width in Musca autumnalis were shown to be influenced by changes in the quality of cattle dung collected in 3 pastures (A, B and C) in eastern-central South Dakota in 1981. The pupal weight and head-capsule width of f...

Rosan, L.; Dascalu, R., 1964: A Case Of Pseudohypoparathyroidism With Secondary Compensating Hyperparathyroidism. Studii Si Cercetari de Endocrinologie 15: 73-76

Soares, Iês.; Goios, A.; Amorim, Aónio., 2013: Sequence comparison alignment-free approach based on suffix tree and L-words frequency. The vast majority of methods available for sequence comparison rely on a first sequence alignment step, which requires a number of assumptions on evolutionary history and is sometimes very difficult or impossible to perform due to the abundance of...

Barbetakis, N.; Asteriou, C.; Vlaikos, D.; Psatha, A., 2010: eComment: Supradiaphragmatic ligation of the thoracic duct for prevention of postoperative chylothorax. Interactive Cardiovascular and Thoracic Surgery 10(1): 139-139

Nita, M.E.lis, M.; Wilson, L.; Madden, L., 2006: Effects of Application of Fungicide During the Dormant Period on Phomopsis Cane and Leaf Spot of Grape Disease Intensity and Inoculum Production. Efficacy of application of the fungicides calcium polysulfide or fixed copper during the dormant period on control of Phomopsis cane and leaf spot of grape (Vitis spp.), caused by Phomopsis viticola, was examined under field conditions during the...

Hamerski, W., 1984: Nocturnal amblyopia. Pielegniarka i Polozna: 5-6

Anonymous, 1999: Honours and Awards--Canadian Society of Agronomy: 1997 Fellow of the CSA: Dr. Bruce E. Coulman. Canadian Journal of Plant Science 79(2): 313

Lin, C.C., 1996: The effect of nasal CPAP on tissue oxygenation in patients with obstructive sleep apnea syndrome. European Respiratory Journal Supplement 9(23): 155S

Nancarrow, J.D., 1996: The use of atraumatic bowel clamps to create a bloodless field in surgery of the lip. Plastic and Reconstructive Surgery 98(7): 1323-1324

Sekine-Osajima, Y.; Nakagawa, M.; Sakamoto, N., 2011: Mechanism of hepatitis C virus (HCV) RNA replication and HCV genetic variability. Nihon Rinsho. Japanese Journal of Clinical Medicine 69 Suppl 4: 52-58