geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Cloning and functional characterization of the rat glutamine synthetase gene


, : Cloning and functional characterization of the rat glutamine synthetase gene. Molecular Brain Research 9(3): 197-208

Glutamine synthetase catalyzes the formation of glutamine from glutamate and ammonia. It plays a central role in both amino acid neurotransmitter metabolism and ammonia detoxification in the central nervous system. Glutamine synthetase expression is regulated in developmental, hormonal, and in tissue- and cell-specific manners. We have cloned a full-length cDNA coding for rat glutamine synthetase, and have found an AT-rich area of conservation in the 3'-untraslanted regions between rat, mouse, and chicken, which may play a part in the regulation of the stability of the glutamine synthetase message. We have also cloned and mapped the gene coding for rat glutamine synthetase, and identified, by sequence analysis, areas potentially important for the regulation of glutamine synthetase transcription. Transient transfection of a variety of cell lines with deletion constructs of the glutamine synthetase promoter driving a chloramphenicol acetyltransferase reporter gene functionally demonstrates regions of the promoter containing elements important for transcriptional regulation.

US$19.90

DOI: 10.1016/0169-328X(91)90003-G


Other references

Salje, E.; Plester, J., 1984: 'Active' noise reduction for circular saws. Studies were made on 'active noise abatement' (i.e., reducing noise emissions directly at their source) in damped and undamped sawblades. The effects of the following factors were analysed: rotation speed and tooth geometry on idling noi...

Anonymous, 2003: Suppliers see growth continuing. Landscape Management 42(9): 5

Huh, S.Y.; Andrew, R.; Rich-Edwards, J.W.; Kleinman, K.P.; Seckl, J.R.; Gillman, M.W., 2008: Association between umbilical cord glucocorticoids and blood pressure at age 3 years. Animal data show that decreased activity of placental 11-beta-hydroxysteroid dehydrogenase type 2 (11beta-HSD2), which potently inactivates glucocorticoids (e.g. cortisol) to inert forms (cortisone), allows increased access of maternal glucocortic...

Prignet, J.M.; Carloz, E.; Kuenkel, D., 1995: Mucosal pseudolipomatosis of the rectosigmoid revealed by a chronic diarrhoea: Pathogenesis discussion about one case and review of the literature. The authors report a case of mucosal pseudolipomatosis of the rectosignoid revealed during investigations of a chronic diarrhoea in a 31 years old man. About this case and upon literature, they discuss the pathogenic mechanisms and the possible im...

Cheung, J.Pui.Yin.; Luk, K.Dip-Kei., 2017: Managing the Pediatric Spine: Growth Assessment. Management of pediatric spinal deformities requires an accurate prediction of growth spurts to allow for timely initiation of treatment and prevention of curve progression. Determining remaining growth potential is also important for avoiding prol...

Batik Petr; Pini Sergio, 1973: Lower Cretaceous and Cenomanian-Turonian strata northeast of Hedil, northern Tunisia. Annales des Mines et de la Geologie 26: 213-218

Hotaling, J.M.; Patel, D.P.; Brant, W.O.; Myers, J.B.; Cullen, M.R.; Eisenberg, M.L., 2015: Demographic and socio-economic differences between men seeking infertility evaluation and those seeking surgical sterilization: from the National Survey of Family Growth. To identify differences in demographic and socio-economic factors between men seeking infertility evaluation and those undergoing vasectomy, to address disparities in access to these services. Data from Cycle 6 and Cycle 7 (2002 and 2006-2008) of...

Volpe, Robert, 1999: Contemporary Endocrinology; Autoimmune endocrinopathies. This book is part of a series of reference works on contemporary endocrinology with an emphasis on the topic of autoimmune endocrinopathies. The seventeen individually authored chapters feature material relevant to autoimmune diseases such as auto...

Wearing, J.; Black, J.; Kline, K., 2011: A model for nurse practitioner regulation: principles underpinning a three-registration category approach. The issue of professional regulation of advanced nursing practice is one of the most controversial in the area of nursing regulation today. The controversy affects the roles and scope of advanced practice as well as education requirements. Nurse p...

Sverckov, O.N. a. N., 1964: The effect of volatile aromatic substances excreted by plants during flowering on the ionization of air. Includes data for the following woody species: Acer negundo, Prunus padus, Robinia pseudoacacia, Salix acutifolia, and Syringa vulgaris. During flowering, the plants affected the ionization of air more than at other times, in proportion to the mas...