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Coexistence of two 24 alkenyl steroids with their 24 alkyl homologue in borage oil

, : Coexistence of two 24 alkenyl steroids with their 24 alkyl homologue in borage oil. Revue Francaise des Corps Gras 36(6): 279-282

Sterols from two borage oils (Borago officinalis L.) were analyzed as acetylated form by GLC on DB1 and DB17 columns and by GLC-MS. Some sterols were isolated by TLC on plates coated with silica gel and AgNO3; their structure was confirmed by 1H NMR. Two 24-alkyl cholest 5-ene 3.beta.-ol, the methyl (26-30%) and the ethyl (22-40%), coexist with their unsaturated precursor in .DELTA.24(28), the methylene (15-20%) and the ethylidene (15-20%). The high levels of the last two compounds constitute the originality of B. officinalis and permit to differentiate this oil from others rich in .gamma.-linolenic acid (evening primrose: oenothera biennis and blackcurrant: Ribes nigrum). It is thus a selected substract for the study of the double bond .DELTA.24(28) reduction step during biosynthesis of these sterols.


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