+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Comparison of different antigen preparations in an evaluation of the immune response to Campylobacter pylori

, : Comparison of different antigen preparations in an evaluation of the immune response to Campylobacter pylori. European Journal of Clinical Microbiology & Infectious Diseases 7(4): 570-575

This study presents a novel approach to the analysis of protein antigens of Campylobacter pylori for use in serology. Protein fractions of this bacterium were resolved in polyacrylamide gel electrophoresis, eluted from gel strips in an electric field and used for coating of microtiter plates in an ELISA-type assay run with a small set of sera from both infected and non-infected patients. Reactivity and discriminative power of the different fractionated antigens (1-9) and crude antigen preparations (A-C) were compared. Better discrimination was achieved between positive and negative sera with high molecular weight fractionated preparations (antigens 8 and 9) than with low molecular weight fractions. Among the crude antigen preparations, antigens A (sonicated whole cells) and C (ultracentrifugated sonicate) seem to have a better discriminative power than antigen B (acid glycin extract).


PMID: 3141175

Other references

Maggio, R.; Ajello, F.; Monroy, A., 1960: Inhibitor of the cytochrome oxidase of unfertilised sea urchin eggs. Nature London, 188: 1195-1196

Seimiya, M.; Kusakabe, T.; Suzuki, N., 1997: Primary structure and differential gene expression of three membrane forms of guanylyl cyclase found in the eye of the teleost Oryzias latipes. Three cDNAs (OlGC3, OlGC4, and OlGC5) encoding membrane guanylyl cyclases were isolated from a medaka (Oryzias latipes) eye cDNA library. An open reading frame for OlGC3 predicted a protein of 1057 amino acids, and those for OlGC4 and OlGC5, 1134...

Margaritov, N., 1987: Parasites on buffalofish: on the bigmouth buffalo (Ictiobus cyprinellus Val.) on the black buffalo (I. niger Raf.) and on their hybrid (I. cyprinellus x I. niger) in fish ponds in Bulgaria. Godishnik na Sofiiskiya Universitet Biologicheski Fakultet. 1984 ; 781: 95-102

Anonymous, 1978: A flat cabinet as an incubator. Tung wu hsueh tsa chih: (2) 36-37

Radike, A.W.; Gish, C.W.; Peterson, J.K.; King, J.D.; Segreto, V.A., 1973: Clinical evaluation of stannous fluoride as an anticaries mouthrinse. Journal of the American Dental Association 86(2): 404-408

Leppik, I.E.; Lapora, J.; Loewenson, R., 1985: Seasonal incidence of phenytoin allergy unrelated to plasma levels. In a three-year prospective study of patients to whom phenytoin was administered, 26 (8.5%) of 306 patients manifested an erythematous morbiliform rash within three weeks of onset of therapy. Occurrence of the rash was not related to the mode of a...

Sastry T.C.S.; Garg S., 1991: Correct authority of cotula hemispherica asteraceae. Journal of Economic & Taxonomic Botany 15(2): 457-458

Shellito, J.G.; Bartlett, W.C., 1958: Diaphragmatic hernia; traumatic, with herniation of tumor of liver. Journal of the Kansas Medical Society 59(2): 51-53

Lin, M-Tsan.; Hsu, C-Sen.; Yeh, S-Ling.; Yeh, C-Li.; Chang, K-Jen.; Lee, P-Huang.; Chen, W-Jao., 2007: Effects of omega-3 fatty acids on leukocyte Th1/Th2 cytokine and integrin expression in rats with gut-derived sepsis. This study examined the effect of fish oil (FO)-enriched diets before and/or omega-3 fatty acid-containing total parenteral nutrition (TPN) after sepsis on the distribution of the T-lymphocyte subpopulation, intracellular cytokine, and intestinal...

Reddy, K.Govardhan.; Deepak, T.G.; Anjusree, G.S.; Thomas, S.; Vadukumpully, S.; Subramanian, K.R.V.; Nair, S.V.; Nair, A.Sreekumaran., 2014: On global energy scenario, dye-sensitized solar cells and the promise of nanotechnology. One of the major problems that humanity has to face in the next 50 years is the energy crisis. The rising population, rapidly changing life styles of people, heavy industrialization and changing landscape of cities have increased energy demands, e...