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Construction of immune rna gene library synthesis of immune rna gene complementary dna


, : Construction of immune rna gene library synthesis of immune rna gene complementary dna. Journal of China Medical University 17(4): 246-248

In order to construct iRNA gene library cDNA was synthesized. The iRNA was extracted from BALB/C mice immunized with E. coli, and through an oligo d(T) chromatography the poly A+ RNA was separated as the template for the cDNA. After alkaline agarose gel electrophoresis and autoradiographic analysis it was shown that the synthesized cDNA had molecular weight of 0.5 .apprx. 2.0kb, which would be suitable for the cDNA library.

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