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Continuous production of erythropoietin with immobilized animal cells


, : Continuous production of erythropoietin with immobilized animal cells. Applied Microbiology & Biotechnology 29(6): 544-549

Erythropoietin, a glycoprotein that is a physiological stimulator of erythrocyte production, was produced continuously for more than 32 days by three kinds of anchorage-dependent animal cells immobilized in alginate gel particles. Gelation caused by divalent cations added to an alginate solution containing cells resulted in the formation of clearly vacant spaces (referred to here as channels) with prolate ellipsoidal shapes inside the gel particles. Each channel originated from a cell and extended towards the center of the gel particle. The animal cells grew well three-dimensionally in the channels but proliferated little outside the channels. Most of the channels had been filled with cells 2 weeks after immobilization. The cell concentration in the gel particles reached more than 1 .times. 107 cells/g gel. The alginate immobilization method was useful for high-concentration cultivation of the anchorage-dependent cells.

US$19.90

DOI: 10.1007/bf00260982


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