+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Continuous production of erythropoietin with immobilized animal cells

, : Continuous production of erythropoietin with immobilized animal cells. Applied Microbiology & Biotechnology 29(6): 544-549

Erythropoietin, a glycoprotein that is a physiological stimulator of erythrocyte production, was produced continuously for more than 32 days by three kinds of anchorage-dependent animal cells immobilized in alginate gel particles. Gelation caused by divalent cations added to an alginate solution containing cells resulted in the formation of clearly vacant spaces (referred to here as channels) with prolate ellipsoidal shapes inside the gel particles. Each channel originated from a cell and extended towards the center of the gel particle. The animal cells grew well three-dimensionally in the channels but proliferated little outside the channels. Most of the channels had been filled with cells 2 weeks after immobilization. The cell concentration in the gel particles reached more than 1 .times. 107 cells/g gel. The alginate immobilization method was useful for high-concentration cultivation of the anchorage-dependent cells.


DOI: 10.1007/bf00260982

Other references

Yang, S.P.; Winyard, P.J.; Woolf, A.S., 2000: Transforming growth factor-beta upregulation in experimental renal dysplasia correlates with cyst formation and an epithelial/mesenchymal switch. Journal of the American Society of Nephrology 11(Program and Abstract Issue): 385A, September

Marwick, T.H.; Shan, K.; Go, R.T.; Macintyre, W.J.; Lauer, M.S.; Underwood, D.A., 1995: Use of rubidium-82 PET for prediction of cardiac events peri- and post-operatively after vascular surgery. Journal of Nuclear Medicine 36(5 SUPPL ): 78P

Ekinci, D.; Al-Rashida, M.; Abbas, G.; Şentürk, M.; Supuran, C.T., 2013: Chromone containing sulfonamides as potent carbonic anhydrase inhibitors. A series of sulfonamide derivatives incorporating substituted 3-formylchromone moieties were investigated for the inhibition of three human carbonic anhydrase (hCA, EC isoforms, hCA I, II, and VI. All these compounds, together with the cl...

Pohl, P.; Lintermans, P.; Moury, J., 1986: Production of aerobactin and its receptor, controlled by R plasmids belonging to incompatibility group FI. 48 R plasmids of Escherichia coli belonged to 21 incompatibility groups, and four (pIP 174, R453, R455 and R456) induced the production of aerobactin in aerobactin-negative strains of E. coli. All four plasmids belonged to group incFI.

Mshvidobadze, N.; Momtselidze, N.; Pagava, K.; Mchedlishvili, G., 2002: Direct in vitro effect of laser beams on erythrocyte aggregation. Biorheology 39(5): 673

Schweitzer, J.B.; Cortes, C.R.; Gullapalli, R.P.; Medoff, D.R.; Dunning, C.; Tagamets, M., 2003: Working memory in children with attention deficit hyperactivity disorder an fMRI study. Both children and adults with attention deficit hyperactivity disorder (ADHD) have deficits in working memory. This functional magnetic resonance imaging (fMRI) study defines the functional neuranatomical pattern of these deficits in children with...

Ohgoe, T.; Suzuki, T.; Kawashima, N., 2012: Commensurate supersolid of three-dimensional lattice bosons. Using an unbiased quantum Monte Carlo method, we obtain convincing evidence of the existence of a checkerboard supersolid at a commensurate filling factor 1/2 (a commensurate supersolid) in the soft-core Bose-Hubbard model with nearest-neighbor r...

Isogai, T.; Ono, H.; Kojo, H., 1997: D-amino acid oxidase from F solani and DNA therefor. Official Gazette of the United States Patent & Trademark Office Patents 1195(2): 1169, Feb 11

Freitag, C.M., 2008: The genetics of autistic disorders. Autistic disorders are heterogeneous. Affected individuals show impairments in communication and social interaction, as well as stereotypic, repetitive behaviour and special interests. The majority of autistic disorders are genetic in origin. The...

Veith, I., 1956: The function and place of the history of medicine in medical education. Journal of Medical Education 31(5): 303-309