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Death of some dorsal root ganglion neurons and plasticity of others following sciatic nerve section in adult and neonatal rats


, : Death of some dorsal root ganglion neurons and plasticity of others following sciatic nerve section in adult and neonatal rats. Journal of Comparative Neurology 284(2): 215-230

Newborn animals recover from neurological injury to a greater extent than adults in spite of the greater vulnerability of developing neurons to retrograde or transneuronal degeneration (Kennard, '42; Goldman, '74; Prendergast and Stelzner, '76; Bregman and Goldberger, '82, '83). The cellular mechanisms underlying this "infant lesion effect" are incompletely understood (Bregman and Goldberger, '82). The dorsal root ganglion (DRG) is an excellent model in which to compare the developing and adult nervous system with respect to the effects of axotomy on cell survival and cellular function. We studied the survival of L5 DRG neurons after section-ligation of the sciatic nerve of adult and neonatal rats and used qualitative and quantitative immunocytochemical methods to examine changes in intraspinal substance P immunoreactivity (SPIR). Retrograde transport of wheatgerm agglutinin-horseradish (WGA-HRP) peroxidase applied to the sciatic nerve of adult or neonatal rats demonstrated that 70% of the neurons in the normal L5 DRG project into the sciatic nerve at the site of transection. In adults 20% of all L5 DRG neurons died between 10 and 60 days postoperative; in newborns 50% of the neurons died between 5 and 10 days. These results indicate that 30% of axotomized neurons in adults and 75% in neonates die after sciatic nerve section and that neuron loss is both more rapid and more extensive in neonates. No cell death was observed in the L5 DRG of neonates after dorsal rhizotomy, thus suggesting that at this stage of development the survival of DRG neurons depends on the peripheral but not the central process. SPIR in laminae I and II of both adult and newborn operates decreased and then recovered, but the time course and extent of the recovery differ. In adults SPIR was depleted in the medial portion of the L5 segment ipsilateral to surgery by 10 days postoperative and remained depleted for at least 2 months. By 1 year partial recovery occurred, but remained incomplete even at the longest survival time studied (15 months). SPIR, which is present in the dorsal horn at birth, was diminished in ipsilateral laminae I and II by 4 days after nerve section on the day of birth. Between 30 days and 60 days, the density of SPIR in the dorsal horn ipsilateral to surgery became virtually indistinguishable from that on the contralateral, intact side, suggesting a more rapid and complete recovery than in adults. The area occupied by SPIR staining remained smaller than that seen on the unoperated side, thus suggesting incomplete or abnormal development of the terminal field in the deafferented dorsal horn. Recutting the sciatic nerve after SPIR recovery depleted the recovered SPIR in both adults and neonates, indicating that in both groups recovery was largely due to DRG neurons that had survived initial transection of their axons. The results indicate that in developing animals 25% of the normal numbers of DRG neurons that survive sciatic nerve section can restore the normal pattern and density of the substance P (SP) projection to the dorsal horn. Immature DRG neurons, therefore, appear to be more likely to die after axotomy, yet those that survive are capable of robust anatomical and biochemical reorganization in comparison to axotomized mature DRG cells.

US$19.90

PMID: 2474003

DOI: 10.1002/cne.902840206


Other references

Gudmundsen, T.E.; Steinsvik, E.D., 1990: Crossed renal ectopia with nephrolithiasis. Crossed unfused renal ectopia is an uncommon anomaly in which one of the uretes crosses the midline from the kidney to the vesical orifice on the opposite side. We report one case of crossed unfused renal ectopia, nephrolithiasis and pain on the s...

Zeiske E., 1974: Morphological and morphometric studies on the olfactory organs of oviparous cyprinodont fishes pisces. Zeitschrift fuer Morphologie der Tiere 77(1): 19-50

Hamscher,G., 2008: Review veterinary medicines in the environment, with particular reference to dust in animal housing. This review covers the ways in which veterinary drugs with antibiotic effects, such as tetracyclines and sulfonamides, to name only two groups of drugs, act as pollutants in the environment. Veterinary drugs are excreted by livestock in urine and...

Koval', E.Z., 1967: New species of entomophilic imperfect fungi from the Maritime Krai 1967 News of the systematics of lower plants. Isaria vassilievae and Hymenostilbe kedrovensis are described.

Meyer-Burg, J., 1972: Laparoscopic examination of the pancreas. Revista Espanola de Las Enfermedades del Aparato Digestivo 38(6): 697-704

Li, H.; Wang, G.; Liu, S.; An, Q.; Zheng, Q.; Li, B.; Li, Z., 2016: Comparative changes in the antioxidant system in the flag leaf of early and normally senescing near-isogenic lines of wheat (Triticum aestivum L.). The antioxidant system was significantly inhibited in the early aging line than the near-isogenic normal aging line during senescence. The antioxidant system plays pivotal roles in removal of reactive oxygen species (ROS) produced during leaf sene...

Frone, M.R., 2000: Interpersonal conflict at work and psychological outcomes: testing a model among young workers. On the basis of A. P. Fiske's (1992) general theory of social relations, a model of interpersonal conflict at work was developed and tested in a sample of young workers. The model predicts that conflict with supervisors is predictive of organ...

Jayaseelan, E.; Rajendran, S.C.; Shariff, S.; Fishbein, D.; Keystone, J.S., 1991: Cutaneous eruptions in Indian tick typhus. Although frequently unrecognized, rickettsial infections may be an important cause for fever and exanthem in persons presenting to physicians in South India. Most often these patients are referred to dermatology departments with a diagnosis of &qu...

Jirasiritham, S.; Sumethkul, V.; Chalermsanyakorn, P.; Jirasiritham, S., 1999: Acute rejection in renal transplantation patient using Cyclosporine vs microemulsion form Cyclosporine. Nephrology Dialysis Transplantation 14(9): A275, Sept

Lerner, T.; Wright, G.; Landes, G., 1992: Probe D16S273 detects a PstI RFLP with 8 alleles. Human Molecular Genetics 1(2): 142-142